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Open AccessArticle

Transcriptome Analyses in Different Cucumber Cultivars Provide Novel Insights into Drought Stress Responses

1
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
2
Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(7), 2067; https://doi.org/10.3390/ijms19072067
Received: 21 May 2018 / Revised: 28 June 2018 / Accepted: 10 July 2018 / Published: 16 July 2018
(This article belongs to the Special Issue Plant Genetics and Molecular Breeding)
Drought stress is one of the most serious threats to cucumber quality and yield. To gain a good understanding of the molecular mechanism upon water deficiency, we compared and analyzed the RNA sequencing-based transcriptomic responses of two contrasting cucumber genotypes, L-9 (drought-tolerant) and A-16 (drought-sensitive). In our present study, combining the analysis of phenotype, twelve samples of cucumber were carried out a transcriptomic profile by RNA-Seq under normal and water-deficiency conditions, respectively. A total of 1008 transcripts were differentially expressed under normal conditions (466 up-regulated and 542 down-regulated) and 2265 transcripts under drought stress (979 up-regulated and 1286 down-regulated). The significant positive correlation between RNA sequencing data and a qRT-PCR analysis supported the results found. Differentially expressed genes (DEGs) involved in metabolic pathway and biosynthesis of secondary metabolism were significantly changed after drought stress. Several genes, which were related to sucrose biosynthesis (Csa3G784370 and Csa3G149890) and abscisic acid (ABA) signal transduction (Csa4M361820 and Csa6M382950), were specifically induced after 4 days of drought stress. DEGs between the two contrasting cultivars identified in our study provide a novel insight into isolating helpful candidate genes for drought tolerance in cucumber. View Full-Text
Keywords: Cucumis sativus L.; RNA-Seq; DEGs; sucrose; ABA; drought stress Cucumis sativus L.; RNA-Seq; DEGs; sucrose; ABA; drought stress
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Wang, M.; Jiang, B.; Peng, Q.; Liu, W.; He, X.; Liang, Z.; Lin, Y. Transcriptome Analyses in Different Cucumber Cultivars Provide Novel Insights into Drought Stress Responses. Int. J. Mol. Sci. 2018, 19, 2067.

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