Comparative Chloroplast Genome Analyses of Species in Gentiana section Cruciata (Gentianaceae) and the Development of Authentication Markers
Abstract
:1. Introduction
2. Results
2.1. Complete Chloroplast Genome Features of Sect. Cruciata
2.2. Comparative Analyses of the Chloroplast Genomes of Species of Sect. Cruciata
2.3. Development of InDel Markers to Discriminate Species of Sect. Cruciata
2.4. Phylogenetic Relationships of Species Belong to Sect. Cruciata
3. Discussion
4. Materials and Methods
4.1. Plant Materials and DNA Isolation
4.2. Chloroplast Genome Sequencing, Assembly and Annotation
4.3. Repeat Structure, Genome Comparison and Sequence Divergence
4.4. Development and Validation of the InDel Molecular Marker
4.5. Phylogenetic Analysis
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Name of Taxon | G. dahurica | G. siphonantha | G. officinalis | G. straminea |
Genome length | 148,803 | 148,908 | 148,879 | 148,991 |
LSC length | 81,154 | 81,121 | 81,119 | 81,240 |
SSC length | 17,093 | 17,113 | 17,088 | 17,085 |
IR length | 25,278 | 25,337 | 25,336 | 25,333 |
Total gene number | 112 | 112 | 112 | 112 |
No. of protein coding genes | 78 | 78 | 78 | 78 |
No. of tRNA genes | 30 | 30 | 30 | 30 |
No. of rRNA genes | 4 | 4 | 4 | 4 |
GC content in genome (%) | 37.7 | 37.7 | 37.7 | 37.7 |
Name of Taxon | G. crassicaulis | G. robusta | G. tibetica | G. macrophylla |
Genome length | 148,776 | 148,911 | 148,765 | 149,916 |
LSC length | 81,164 | 81,164 | 81,163 | 82,911 |
SSC length | 17,071 | 17,085 | 17,070 | 17,095 |
IR length | 25,271 | 25,333 | 25,266 | 24,955 |
Total gene number | 112 | 112 | 112 | 112 |
No. of protein coding genes | 78 | 78 | 78 | 78 |
No. of tRNA genes | 30 | 30 | 30 | 30 |
No. of rRNA genes | 4 | 4 | 4 | 4 |
GC content in genome (%) | 37.7 | 37.7 | 37.7 | 37.7 |
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Zhou, T.; Wang, J.; Jia, Y.; Li, W.; Xu, F.; Wang, X. Comparative Chloroplast Genome Analyses of Species in Gentiana section Cruciata (Gentianaceae) and the Development of Authentication Markers. Int. J. Mol. Sci. 2018, 19, 1962. https://doi.org/10.3390/ijms19071962
Zhou T, Wang J, Jia Y, Li W, Xu F, Wang X. Comparative Chloroplast Genome Analyses of Species in Gentiana section Cruciata (Gentianaceae) and the Development of Authentication Markers. International Journal of Molecular Sciences. 2018; 19(7):1962. https://doi.org/10.3390/ijms19071962
Chicago/Turabian StyleZhou, Tao, Jian Wang, Yun Jia, Wenli Li, Fusheng Xu, and Xumei Wang. 2018. "Comparative Chloroplast Genome Analyses of Species in Gentiana section Cruciata (Gentianaceae) and the Development of Authentication Markers" International Journal of Molecular Sciences 19, no. 7: 1962. https://doi.org/10.3390/ijms19071962