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Int. J. Mol. Sci. 2018, 19(6), 1728; https://doi.org/10.3390/ijms19061728

Gene Profiling in Late Blight Resistance in Potato Genotype SD20

1
Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable, Jinan 250100, China
2
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, Beijing 100081, China
3
National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 15 April 2018 / Revised: 18 May 2018 / Accepted: 4 June 2018 / Published: 11 June 2018
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Abstract

Late blight caused by the oomycete fungus Phytophthora infestans (Pi) is the most serious obstacle to potato (Solanum tuberosum) production in the world. A super race isolate, CN152, which was identified from Sichuan Province, China, could overcome nearly all known late blight resistance genes and caused serious damage in China. The potato genotype SD20 was verified to be highly resistant to CN152; however, the molecular regulation network underlying late blight resistance pathway remains unclear in SD20. Here, we performed a time-course experiment to systematically profile the late blight resistance response genes using RNA-sequencing in SD20. We identified 3354 differentially expressed genes (DEGs), which mainly encoded transcription factors and protein kinases, and also included four NBS-LRR genes. The late blight responsive genes showed time-point-specific induction/repression. Multi-signaling pathways of salicylic acid, jasmonic acid, and ethylene signaling pathways involved in resistance and defense against Pi in SD20. Gene Ontology and KEGG analyses indicated that the DEGs were significantly enriched in metabolic process, protein serine/threonine kinase activity, and biosynthesis of secondary metabolites. Forty-three DEGs were involved in immune response, of which 19 were enriched in hypersensitive response reaction, which could play an important role in broad-spectrum resistance to Pi infection. Experimental verification confirmed the induced expression of the responsive genes in the late blight resistance signaling pathway, such as WRKY, ERF, MAPK, and NBS-LRR family genes. Our results provided valuable information for understanding late blight resistance mechanism of potato. View Full-Text
Keywords: potato; late blight; Phytophthora infestans; transcriptome; resistance potato; late blight; Phytophthora infestans; transcriptome; resistance
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Yang, X.; Guo, X.; Yang, Y.; Ye, P.; Xiong, X.; Liu, J.; Dong, D.; Li, G. Gene Profiling in Late Blight Resistance in Potato Genotype SD20. Int. J. Mol. Sci. 2018, 19, 1728.

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