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Open AccessArticle

Mechanism of Salt-Induced Self-Compatibility Dissected by Comparative Proteomic Analysis in Brassica napus L.

National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, China
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(6), 1652;
Received: 2 May 2018 / Revised: 29 May 2018 / Accepted: 30 May 2018 / Published: 3 June 2018
(This article belongs to the Collection Advances in Proteomic Research)
Self-incompatibility (SI) in plants genetically prevents self-fertilization to promote outcrossing and genetic diversity. Its hybrids in Brassica have been widely cultivated due to the propagation of SI lines by spraying a salt solution. We demonstrated that suppression of Brassica napus SI from edible salt solution treatment was ascribed to sodium chloride and independent of S haplotypes, but it did not obviously change the expression of SI-related genes. Using the isobaric tags for relative and absolute quantitation (iTRAQ) technique, we identified 885 differentially accumulated proteins (DAPs) in Brassica napus stigmas of un-pollinated (UP), pollinated with compatible pollen (PC), pollinated with incompatible pollen (PI), and pollinated with incompatible pollen after edible salt solution treatment (NA). Of the 307 DAPs in NA/UP, 134 were unique and 94 were shared only with PC/UP. In PC and NA, some salt stress protein species, such as glyoxalase I, were induced, and these protein species were likely to participate in the self-compatibility (SC) pathway. Most of the identified protein species were related to metabolic pathways, biosynthesis of secondary metabolites, ribosome, and so on. A systematic analysis implied that salt treatment-overcoming SI in B. napus was likely conferred by at least five different physiological mechanisms: (i) the use of Ca2+ as signal molecule; (ii) loosening of the cell wall to allow pollen tube penetration; (iii) synthesis of compatibility factor protein species for pollen tube growth; (iv) depolymerization of microtubule networks to facilitate pollen tube movement; and (v) inhibition of protein degradation pathways to restrain the SI response. View Full-Text
Keywords: Brassica napus; iTRAQ; self-incompatibility; self-compatibility; stigma; salt Brassica napus; iTRAQ; self-incompatibility; self-compatibility; stigma; salt
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Yang, Y.; Liu, Z.; Zhang, T.; Zhou, G.; Duan, Z.; Li, B.; Dou, S.; Liang, X.; Tu, J.; Shen, J.; Yi, B.; Fu, T.; Dai, C.; Ma, C. Mechanism of Salt-Induced Self-Compatibility Dissected by Comparative Proteomic Analysis in Brassica napus L.. Int. J. Mol. Sci. 2018, 19, 1652.

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