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Int. J. Mol. Sci. 2018, 19(6), 1593; https://doi.org/10.3390/ijms19061593

Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza

1,2,3,4,†, 2,3,†, 2,3, 2,3 and 1,2,3,4,*
1
Institute of Soil and Water Conservation, Chinese Academy of Sciences (CAS) & Ministry of Water Resources (MWR), Yangling 712100, China
2
Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, Zhejiang Sci-Tech University, Hangzhou 310018, China
3
College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
4
University of Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 19 April 2018 / Revised: 18 May 2018 / Accepted: 24 May 2018 / Published: 29 May 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Salvia miltiorrhiza Bunge is a Chinese traditional herb for treating cardiovascular and cerebrovascular diseases, and tanshinones and phenolic acids are the dominated medicinal and secondary metabolism constituents of this plant. WRKY transcription factors (TFs) can function as regulators of secondary metabolites biosynthesis in many plants. However, studies on the WRKY that regulate tanshinones and phenolics biosynthesis are limited. In this study, 69 SmWRKYs were identified in the transcriptome database of S. miltiorrhiza, and phylogenetic analysis indicated that some SmWRKYs had closer genetic relationships with other plant WRKYs, which were involved in secondary metabolism. Hairy roots of S. miltiorrhiza were treated by methyl jasmonate (MeJA) to detect the dynamic change trend of SmWRKY, biosynthetic genes, and medicinal ingredients accumulation. Base on those date, a correlation analysis using Pearson’s correlation coefficient was performed to construct gene-to-metabolite network and identify 9 SmWRKYs (SmWRKY1, 7, 19, 29, 45, 52, 56, 58, and 68), which were most likely to be involved in tanshinones and phenolic acids biosynthesis. Taken together, this study has provided a significant resource that could be used for further research on SmWRKY in S. miltiorrhiza and especially could be used as a cue for further investigating SmWRKY functions in secondary metabolite accumulation. View Full-Text
Keywords: Salvia miltiorrhiza; SmWRKY; secondary metabolites; tanshinones; phenolic acid Salvia miltiorrhiza; SmWRKY; secondary metabolites; tanshinones; phenolic acid
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Yu, H.; Guo, W.; Yang, D.; Hou, Z.; Liang, Z. Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza. Int. J. Mol. Sci. 2018, 19, 1593.

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