Next Article in Journal
The Spleen as an Optimal Site for Islet Transplantation and a Source of Mesenchymal Stem Cells
Previous Article in Journal
Cancer Immunotherapy: A Focus on the Regulation of Immune Checkpoints
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle

Preanalytical Biases in the Measurement of Human Blood Sphingolipids

1
Department of Neurology, Goethe University Hospital Frankfurt, 60590 Frankfurt am Main, Germany
2
Department of General Pharmacology and Toxicology, Goethe University Hospital Frankfurt, 60590 Frankfurt am Main, Germany
3
Department of Nephrology, Goethe University Hospital Frankfurt, 60590 Frankfurt am Main, Germany
4
Department of Clinical Pharmacology, Goethe University Hospital Frankfurt, 60590 Frankfurt am Main, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(5), 1390; https://doi.org/10.3390/ijms19051390
Received: 16 March 2018 / Revised: 15 April 2018 / Accepted: 3 May 2018 / Published: 7 May 2018
(This article belongs to the Section Biochemistry)
  |  
PDF [2274 KB, uploaded 24 May 2018]
  |  

Abstract

Dysregulation of blood sphingolipids is an emerging topic in clinical science. The objective of this study was to determine preanalytical biases that typically occur in clinical and translational studies and that influence measured blood sphingolipid levels. Therefore, we collected blood samples from four healthy male volunteers to investigate the effect of storage conditions (time, temperature, long-term storage, freeze–thaw cycles), blood drawing (venous or arterial sampling, prolonged venous compression), and sample preparation (centrifugation, freezing) on sphingolipid levels measured by LC-MS/MS. Our data show that sphingosine 1-phosphate (S1P) and sphinganine 1-phosphate (SA1P) were upregulated in whole blood samples in a time- and temperature-dependent manner. Increased centrifugation at higher speeds led to lower amounts of S1P and SA1P. All other preanalytical biases did not significantly alter the amounts of S1P and SA1P. Further, in almost all settings, we did not detect differences in (dihydro)ceramide levels. In summary, besides time-, temperature-, and centrifugation-dependent changes in S1P and SA1P levels, sphingolipids in blood remained stable under practically relevant preanalytical conditions. View Full-Text
Keywords: sphingolipids; sphingosine 1-phosphate; sphinganine 1-phosphate; ceramides; dihydroceramides; blood sphingolipids; sphingosine 1-phosphate; sphinganine 1-phosphate; ceramides; dihydroceramides; blood
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Brunkhorst, R.; Pfeilschifter, W.; Patyna, S.; Büttner, S.; Eckes, T.; Trautmann, S.; Thomas, D.; Pfeilschifter, J.; Koch, A. Preanalytical Biases in the Measurement of Human Blood Sphingolipids. Int. J. Mol. Sci. 2018, 19, 1390.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top