Next Article in Journal
The Interaction of the Metallo-Glycopeptide Anti-Tumour Drug Bleomycin with DNA
Next Article in Special Issue
CaWRKY40b in Pepper Acts as a Negative Regulator in Response to Ralstonia solanacearum by Directly Modulating Defense Genes Including CaWRKY40
Previous Article in Journal
The Effect of Bornyl cis-4-Hydroxycinnamate on Melanoma Cell Apoptosis Is Associated with Mitochondrial Dysfunction and Endoplasmic Reticulum Stress
Previous Article in Special Issue
The Commonly Used Bactericide Bismerthiazol Promotes Rice Defenses against Herbivores
Open AccessArticle

A Biocontrol Strain of Bacillus subtilis WXCDD105 Used to Control Tomato Botrytis cinerea and Cladosporium fulvum Cooke and Promote the Growth of Seedlings

1
College of Life Sciences, Northeast Agricultural University, 150030 Harbin, China
2
College of Horticulture and Landscape Architecture, Northeast Agricultural University, 150030 Harbin, China
3
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, 150030 Harbin, China
4
College of Agronomy, Northeast Agricultural University, 150030 Harbin, China
5
College of Science, Northeast Agricultural University, 150030 Harbin, China
6
Institute of Microbiology Heilongjiang Academy of Sciences, 150001 Harbin, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(5), 1371; https://doi.org/10.3390/ijms19051371
Received: 5 April 2018 / Revised: 27 April 2018 / Accepted: 30 April 2018 / Published: 4 May 2018
(This article belongs to the Special Issue Plant Innate Immunity 2.0)
In this study, a strain named WXCDD105, which has strong antagonistic effects on Botrytis cinerea and Cladosporium fulvum Cooke, was screened out from the rhizosphere of healthy tomato plants. The tomato plants had inhibition diameter zones of 5.00 mm during the dual culture for four days. Based on the morphological and physiological characteristics, the 16S rDNA sequence, and the gyrB gene sequence analysis, the strain WXCDD105 was identified as Bacillus subtilis suBap. subtilis. The results of the mycelial growth test showed that the sterile filtrate of the strain WXCDD105 could significantly inhibit mycelial growth of Botrytis cinerea and Cladosporium fulvum Cooke. The inhibition rates were 95.28 and 94.44%, respectively. The potting experiment showed that the strain WXCDD105 made effective the control of tomato gray mold and tomato leaf mold. The control efficiencies were 74.70 and 72.07%. The antagonistic test results showed that the strain WXCDD105 had different degrees of inhibition on 10 kinds of plant pathogenic fungi and the average inhibition rates were more than 80%. We also found that the strain WXCDD105 stimulated both the seed germination and seedling growth of tomatoes. Using the fermentation liquid of WXCDD105 (108 cfu·mL−1) to treat the seeds, the germination rate and radicle length were increased. Under the treatment of the fermentation liquid of the strain WXCDD105 (106 cfu·mL−1), nearly all physiological indexes of tomato seedlings were significantly higher than that of the control groups. This could not only keep the nutritional quality of tomato fruits but also prevent them from rotting. This study provided us with an excellent strain for biological control of tomato gray mold, tomato leaf mold, and tomato growth promotion. This also laid the technical foundation for its application. View Full-Text
Keywords: tomato gray mold; tomato leaf mold; Bacillus subtilis; biological control tomato gray mold; tomato leaf mold; Bacillus subtilis; biological control
Show Figures

Graphical abstract

MDPI and ACS Style

Wang, H.; Shi, Y.; Wang, D.; Yao, Z.; Wang, Y.; Liu, J.; Zhang, S.; Wang, A. A Biocontrol Strain of Bacillus subtilis WXCDD105 Used to Control Tomato Botrytis cinerea and Cladosporium fulvum Cooke and Promote the Growth of Seedlings. Int. J. Mol. Sci. 2018, 19, 1371.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop