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Open AccessArticle

α-Ketoglutarate Promotes Pancreatic Progenitor-Like Cell Proliferation

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Screening, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China
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Int. J. Mol. Sci. 2018, 19(4), 943; https://doi.org/10.3390/ijms19040943
Received: 11 February 2018 / Revised: 12 March 2018 / Accepted: 15 March 2018 / Published: 22 March 2018
(This article belongs to the Section Biochemistry)
A major source of β cell generation is pancreatic progenitor-like cell differentiation. Multiple studies have confirmed that stem cell metabolism plays important roles in self-renewal and proliferation. In the absence of glucose, glutamine provides the energy for cell division and growth. Furthermore, α-ketoglutarate (αKG), a precursor for glutamine synthesis, is sufficient for enabling glutamine-independent cell proliferation. We have demonstrated that αKG contributes to the large-scale proliferation of pancreatic progenitor-like cells that can provide an ample amount of clinically relevant β cells. We compared the mRNA expression of a subset of genes, the abundance of ATP, reactive oxide species, mitochondrial number, and the colony-forming frequency between mouse pancreatic CD133+ and CD133 cells. We employed Real-Time PCR, immunostaining and passage assays to investigate self-renewal and proliferation of pancreatic progenitor-like cells in a 3D culture system in the presence and absence of αKG. The energy metabolism of CD133+ cells was more prone to oxidative phosphorylation. However, in the 3D culture system, when αKG was supplemented to the culture medium, the proliferation of the pancreatic progenitor-like cells was significantly elevated. We confirmed that the presence of αKG correlated with the up-regulation of Ten-Eleven Translocation (Tet). αKG can promote the proliferation of pancreatic progenitor-like cells via the up-regulation of Tet. View Full-Text
Keywords: pancreatic progenitor-like cells; α-ketoglutarate; proliferation pancreatic progenitor-like cells; α-ketoglutarate; proliferation
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MDPI and ACS Style

Song, J.; Ma, D.; Xing, Y.; Tang, S.; Alahdal, M.; Guo, J.; Pan, Y.; Zhang, Y.; Shen, Y.; Wu, Q.; Lu, Z.; Jin, L. α-Ketoglutarate Promotes Pancreatic Progenitor-Like Cell Proliferation. Int. J. Mol. Sci. 2018, 19, 943. https://doi.org/10.3390/ijms19040943

AMA Style

Song J, Ma D, Xing Y, Tang S, Alahdal M, Guo J, Pan Y, Zhang Y, Shen Y, Wu Q, Lu Z, Jin L. α-Ketoglutarate Promotes Pancreatic Progenitor-Like Cell Proliferation. International Journal of Molecular Sciences. 2018; 19(4):943. https://doi.org/10.3390/ijms19040943

Chicago/Turabian Style

Song, Jing; Ma, Dongshen; Xing, Yun; Tang, Shanshan; Alahdal, Murad; Guo, Jiamin; Pan, Yi; Zhang, Yanfeng; Shen, Yumeng; Wu, Qiong; Lu, Zhou; Jin, Liang. 2018. "α-Ketoglutarate Promotes Pancreatic Progenitor-Like Cell Proliferation" Int. J. Mol. Sci. 19, no. 4: 943. https://doi.org/10.3390/ijms19040943

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