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Int. J. Mol. Sci. 2018, 19(4), 1142; https://doi.org/10.3390/ijms19041142

Atomic Force Microscopy for Protein Detection and Their Physicoсhemical Characterization

Institute of Biomedical Chemistry, 10, Pogodinskaya St., 119121 Moscow, Russia
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Received: 26 February 2018 / Revised: 30 March 2018 / Accepted: 5 April 2018 / Published: 10 April 2018
(This article belongs to the Special Issue Atomic Force Microscopy for Biological Applications)
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Abstract

This review is focused on the atomic force microscopy (AFM) capabilities to study the properties of protein biomolecules and to detect the proteins in solution. The possibilities of application of a wide range of measuring techniques and modes for visualization of proteins, determination of their stoichiometric characteristics and physicochemical properties, are analyzed. Particular attention is paid to the use of AFM as a molecular detector for detection of proteins in solutions at low concentrations, and also for determination of functional properties of single biomolecules, including the activity of individual molecules of enzymes. Prospects for the development of AFM in combination with other methods for studying biomacromolecules are discussed. View Full-Text
Keywords: atomic force microscopy; protein fishing; protein characterization atomic force microscopy; protein fishing; protein characterization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Pleshakova, T.O.; Bukharina, N.S.; Archakov, A.I.; Ivanov, Y.D. Atomic Force Microscopy for Protein Detection and Their Physicoсhemical Characterization. Int. J. Mol. Sci. 2018, 19, 1142.

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