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Int. J. Mol. Sci. 2018, 19(4), 1095; https://doi.org/10.3390/ijms19041095

Global Transcriptome Analysis of Brown Adipose Tissue of Diet-Induced Obese Mice

1
Program of Physiology and Neuroscience, Department of Biology, Miami University, Oxford, OH 45056, USA
2
Program of Bioinformatics, Department of Biology, Miami University, Oxford, OH 45056, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 16 February 2018 / Revised: 17 March 2018 / Accepted: 24 March 2018 / Published: 6 April 2018
(This article belongs to the Special Issue Nutrition, Brown and White Adipose Tissue)
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Abstract

Consumption of a high-fat diet (HFD) promotes the development of obesity, a disease resulting from an imbalance between energy intake and energy expenditure. Brown adipose tissue (BAT) has thermogenic capacity that burns calories to produce heat, and it is a potential target for the treatment and prevention of obesity. There is limited information regarding the impact of HFD on the BAT transcriptome. We hypothesized that HFD-induced obesity would lead to transcriptional regulation of BAT genes. RNA sequencing was used to generate global transcriptome profiles from BAT of lean mice fed with a low-fat diet (LFD) and obese mice fed with a HFD. Gene Ontology (GO) analysis identified increased expression of genes involved in biological processes (BP) related to immune responses, which enhanced molecular function (MF) in chemokine activity; decreased expression of genes involved in BP related to ion transport and muscle structure development, which reduced MF in channel and transporter activity and structural binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional pathway analysis indicated that pathways associated with innate immunity were enhanced by HFD, while pathways associated with muscle contraction and calcium signaling were suppressed by HFD. Collectively, these results suggest that diet-induced obesity changes transcriptomic signatures of BAT, leading to dysfunction involving inflammation, calcium signaling, ion transport, and cell structural development. View Full-Text
Keywords: high-fat diet; RNA sequencing; brown adipose tissue; muscle system process; inflammation; calcium signaling; ion transport high-fat diet; RNA sequencing; brown adipose tissue; muscle system process; inflammation; calcium signaling; ion transport
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Cao, J.; Zhu, Q.; Liu, L.; Glazier, B.J.; Hinkel, B.C.; Liang, C.; Shi, H. Global Transcriptome Analysis of Brown Adipose Tissue of Diet-Induced Obese Mice. Int. J. Mol. Sci. 2018, 19, 1095.

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