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Int. J. Mol. Sci. 2018, 19(4), 1014; https://doi.org/10.3390/ijms19041014

Probing Interactions between AuNPs/AgNPs and Giant Unilamellar Vesicles (GUVs) Using Hyperspectral Dark-field Microscopy

1
Department of Physics and Engineering, Delaware State University, 1200 N. DuPont Hwy, Dover, DE 19977, USA
2
School of Science, Changchun University of Science and Technology, 7089 Weixing Rd, Changchun 130022, China
*
Author to whom correspondence should be addressed.
Received: 16 February 2018 / Revised: 17 March 2018 / Accepted: 20 March 2018 / Published: 28 March 2018
(This article belongs to the Special Issue Nanotechnology in Cancer Treatment)
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Abstract

Noble metallic nanoparticles (NPs) such as gold and silver nanoparticles (AuNPs and AgNPs) have been shown to exhibit anti-tumor effect in anti-angiogenesis, photothermal and radio therapeutics. On the other hand, cell membranes are critical locales for specific targeting of cancerous cells. Therefore, NP-membrane interactions need be studied at molecular level to help better understand the underlying physicochemical mechanisms for future applications in cancer nanotechnology. Herein, we report our study on the interactions between citrate stabilized colloidal AuNPs/AgNPs (10 nm in size) and giant unilamellar vesicles (GUVs) using hyperspectral dark-field microscopy. GUVs are large model vesicle systems well established for the study of membrane dynamics. GUVs used in this study were prepared with dimyristoyl phosphatidylcholine (DMPC) and doped with cholesterol at various molar concentrations. Both imaging and spectral results support that AuNPs and AgNPs interact very differently with GUVs, i.e., AuNPs tend to integrate in between the lipid bilayer and form a uniform golden-brown crust on vesicles, whereas AgNPs are bejeweled on the vesicle surface as isolated particles or clusters with much varied configurations. The more disruptive capability of AuNPs is hypothesized to be responsible for the formation of golden brown crusts in AuNP-GUV interaction. GUVs of 20 mol% CHOL:DMPC were found to be a most economical concentration for GUVs to achieve the best integrity and the least permeability, consistent with the finding from other phase studies of lipid mixture that the liquid-ordered domains have the largest area fraction of the entire membrane at around 20 mol% of cholesterol. View Full-Text
Keywords: gold nanoparticles; silver nanoparticles; giant unilamellar vesicles; dark-field microscopy; hyperspectral imaging; DMPC; cholesterol gold nanoparticles; silver nanoparticles; giant unilamellar vesicles; dark-field microscopy; hyperspectral imaging; DMPC; cholesterol
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Bhat, A.; Huan, K.; Cooks, T.; Boukari, H.; Lu, Q. Probing Interactions between AuNPs/AgNPs and Giant Unilamellar Vesicles (GUVs) Using Hyperspectral Dark-field Microscopy. Int. J. Mol. Sci. 2018, 19, 1014.

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