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Int. J. Mol. Sci. 2018, 19(3), 783;

miR-146b Inhibits Glucose Consumption by Targeting IRS1 Gene in Porcine Primary Adipocytes

College of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, South China Agricultural University, Guangzhou 510642, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 2 February 2018 / Revised: 27 February 2018 / Accepted: 27 February 2018 / Published: 9 March 2018
(This article belongs to the Section Biochemistry)
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Adipose tissue plays an important role in energy metabolism. Adipose dysfunction is closely related to obesity and type II diabetes. Glucose uptake is the key step for fat synthesis in adipocyte. miRNAs have been proven to play a crucial role in adipocyte differentiation, adipogenesis and glucose homeostasis. In this paper, we firstly reported that miR-146b decreased glucose consumption by up-regulating miR-146b in a porcine primary adipocyte model, while the inhibitor of endogenous miR-146b rescued the reduction. Then, miR-146b was predicated to target IRS1 by bioinformatics analysis, and a dual-luciferase reporter assay validated this predication. Western blot analyses indicated both IRS1 and glucose transporter type 4 (GLUT4) were down-regulated by miR-146b overexpression. Our study demonstrated that miR-146b regulated glucose homeostasis in porcine primary pre-adipocyte by targeting IRS1, and provided new understandings on regulations of lipogenesis by miRNAs. View Full-Text
Keywords: miR-146b; glucose consumption; adipocyte; GLUT4; IRS1 miR-146b; glucose consumption; adipocyte; GLUT4; IRS1

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Zhu, Y.-L.; Chen, T.; Xiong, J.-L.; Wu, D.; Xi, Q.-Y.; Luo, J.-Y.; Sun, J.-J.; Zhang, Y.-L. miR-146b Inhibits Glucose Consumption by Targeting IRS1 Gene in Porcine Primary Adipocytes. Int. J. Mol. Sci. 2018, 19, 783.

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