Fine Physical Bin Mapping of the Powdery Mildew Resistance Gene Pm21 Based on Chromosomal Structural Variations in Wheat
Abstract
:1. Introduction
2. Results
2.1. Physical Mapping of Pm21
2.2. Comparative Genomics Analysis of 6VS FLb4–b5/b6
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Development of 6VS-Specific Markers
4.3. DNA Extraction and PCR
4.4. Physical Mapping of Pm21
4.5. Comparative Genomics Analysis of the Pm21 Locus
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Genetic Stock | Treatment | Chromosomal Breakpoint | Flanking Markers |
---|---|---|---|
del.6VS-1 | Irradiation a | b2 (FL0.58) | 6VS-03/6VS-04 |
b6 | 6VS-10/6VS-10.2 | ||
b7 | 6VS-10.8/6VS-11 | ||
del.6VS-2 | Irradiation | b9 (FL0.45) | CINAU15m/6VS-23 |
NAU418 | Irradiation | b4 | 6VS-08.6/6VS-08.8 |
b8 | 6VS-13/6VS-13.5 | ||
NAU419 | Irradiation | b1 | Xcinau188/6VS-01 |
b10 (Close to b9) | CINAU15m/6VS-23 | ||
Y18-S6 | EMS | b3 (Close to b2) | 6VS-03/6VS-04 |
b5 (Close to b6) | 6VS-10/6VS-10.2 |
Marker | Brachypodium | Rice | Wheat | Gene Annotation |
---|---|---|---|---|
6VS-08.6 | Bradi3g03850 | 6AS_contigs_4399884 6BS_contigs_2953789 6DS_contigs_2117578 | Eukaryotic translation initiation factor | |
6VS-08.8 | Bradi3g03860 | LOC_Os02g05620 | 6AS_contigs_4431592 6BS_contigs_2953283 6DS_contigs_2092656 | Exocyst complex subunit EXO70-like protein |
6VS-09 | Bradi3g03870 | LOC_Os02g05630 | 6AS_contigs_4363243 6BS_contigs_2962596 6DS_contigs_2093935 | Serine/threonine protein phosphatase 2C |
MBH1 | Bradi3g03874 a | Six contigs a | Disease resistance protein | |
6VS-09.4 | Bradi3g03878 a | Six contigs a | Disease resistance protein | |
Bradi3g03882 a | Six contigs a | Disease resistance protein | ||
Bradi3g03886 | Unknown protein | |||
Bradi3g03890 | Unknown protein | |||
Bradi3g03900 | Unknown protein | |||
Bradi3g03910 | Cytochrome P450 | |||
Bradi3g03920 | Unknown protein | |||
Bradi3g03930 | Poly(A) polymerase | |||
Bradi3g03935 a | Six contigs a | Disease resistance protein | ||
Bradi3g03940 | 6AS_contigs_4428294 6BS_contigs_2926507 6DS_contigs_2114667 | Photosystem II protein J | ||
Bradi3g03945 | 6AS_contigs_4428294 6BS_contigs_2926507 6DS_contigs_2114667 | Photosystem II cytochrome b559 alpha subunit | ||
Bradi3g03950 | Unknown protein | |||
Bradi3g03957 | 6AS_contigs_4431958 6BS_contigs_2953283 6DS_contigs_2081863 | Polyubiquitin | ||
6VS-10 | Bradi3g03970 | LOC_Os02g05640 | 6AS_contigs_4429249 b 6BS_contigs_1424756 b | Homeobox-leucine zipper protein HOX26 |
6DS_contigs_2087435 b | ||||
LOC_Os02g05650 | Unknown protein | |||
Bradi3g03980 | 6AS_contigs_2062052 | Unknown protein | ||
6DS_contigs_2110435 | ||||
Bradi3g03990 | LOC_Os02g05660 | DEAD-box ATP-dependent RNA helicase | ||
6VS-10.2 | Bradi3g04000 | LOC_Os02g05661 | 6AS_contigs_4428740 6DS_contigs_2082952 | Unknown protein |
Marker | Strategy | Primer Sequence (5′ → 3′) | Annealing Temperature (°C) | Size of Polymorphic Band (bp) |
---|---|---|---|---|
6VS-08.6 | CISP-IS | F: GATCTCGTTTTAGTCCTGAGCCTGT | 60 | 604 |
R: CCGAGTGAAGTCTGCTCATCCAGT | ||||
6VS-12.5 | CISP | F: CCGGCGACGCGCACTAC | 60 | 391 |
R: GTACTTGTCCGCGAACTCGAAC | ||||
6VS-13.5 | CISP | F: TGCCTCATCACCCCAGTGAA | 60 | 287 |
R: TGCAGTAGGTGGCATAGAGCAAG |
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Zhu, S.; Ji, Y.; Ji, J.; Bie, T.; Gao, A.; He, H. Fine Physical Bin Mapping of the Powdery Mildew Resistance Gene Pm21 Based on Chromosomal Structural Variations in Wheat. Int. J. Mol. Sci. 2018, 19, 643. https://doi.org/10.3390/ijms19020643
Zhu S, Ji Y, Ji J, Bie T, Gao A, He H. Fine Physical Bin Mapping of the Powdery Mildew Resistance Gene Pm21 Based on Chromosomal Structural Variations in Wheat. International Journal of Molecular Sciences. 2018; 19(2):643. https://doi.org/10.3390/ijms19020643
Chicago/Turabian StyleZhu, Shanying, Yaoyong Ji, Jian Ji, Tongde Bie, Anli Gao, and Huagang He. 2018. "Fine Physical Bin Mapping of the Powdery Mildew Resistance Gene Pm21 Based on Chromosomal Structural Variations in Wheat" International Journal of Molecular Sciences 19, no. 2: 643. https://doi.org/10.3390/ijms19020643