The In Vitro Effects of Enzymatic Digested Gliadin on the Functionality of the Autophagy Process
Manai, F.; Azzalin, A.; Gabriele, F.; Martinelli, C.; Morandi, M.; Biggiogera, M.; Bozzola, M.; Comincini, S. The In Vitro Effects of Enzymatic Digested Gliadin on the Functionality of the Autophagy Process. Int. J. Mol. Sci. 2018, 19, 635.
Manai F, Azzalin A, Gabriele F, Martinelli C, Morandi M, Biggiogera M, Bozzola M, Comincini S. The In Vitro Effects of Enzymatic Digested Gliadin on the Functionality of the Autophagy Process. International Journal of Molecular Sciences. 2018; 19(2):635.Chicago/Turabian Style
Manai, Federico; Azzalin, Alberto; Gabriele, Fabio; Martinelli, Carolina; Morandi, Martina; Biggiogera, Marco; Bozzola, Mauro; Comincini, Sergio. 2018. "The In Vitro Effects of Enzymatic Digested Gliadin on the Functionality of the Autophagy Process." Int. J. Mol. Sci. 19, no. 2: 635.
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Description: Supplementary Figure 1 LC3 and Lamp1 expression in Caco-2 cells after PT-gliadin administration. (A,B) Immunofluorescence analysis of LC3 or Lamp1 (green) and gliadin (red) expression, visualized using an inverted microscope Eclipse Nikon TS100, 100X oil immersion Plan Flor objective. Scale bars=10 m. (C) Immunoblotting expression and densitometric analysis of Lamp1 normalized with BACT housekeeping values. Asterisks indicate p<0.05, Anova One-way, compared to T0 untreated sample. Supplementary Figure 2. Micronuclei formation after PT-gliadin administration in Caco-2 cells. Fluorescent DAPI staining and analysis of the number of micronuclei in Caco-2 cells treated with PT-gliadin (1 g/l). For each condition, 1000 nuclei were considered. Scale bars=10 m. Asterisks indicates p<0.05, Anova One-way, compared to NT untreated samples. Supplementary Figure 3. Effect of BECN1 silencing on Caco-2 cells after PT-gliadin administration. PT-gliadin (1 g/l) was administered to Caco-2 cells, transfected with a pool of validated siBECN1 molecules. (A) Immunoblotting and densitometric analysis of BECN1 protein expression. (B) Collected media were analysed by fluorimeter (ext. 492 nm – emis. 517 nm). Asterisk indicates statistical significance p<0.05, Anova One-way, compared to untreated sample (nt). Fluorescence was reported as arbitrary units. SD bars (n=3) are reported.