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Int. J. Mol. Sci. 2018, 19(2), 550; https://doi.org/10.3390/ijms19020550

Expression of Pluripotency Genes in Chondrocyte-Like Cells Differentiated from Human Induced Pluripotent Stem Cells

1
Radiobiology Lab, Greater Poland Cancer Centre, Garbary 15th Street, 61-866 Poznan, Poland
2
The Postgraduate School of Molecular Medicine, Medical University of Warsaw, Zwirki i Wigury 61 Street, 02-091 Warsaw, Poland
3
Department of Electroradiology, Poznan University of Medical Sciences, Garbary 15th Street, 61-866 Poznan, Poland
4
Department of Histology and Embryology, Poznan University of Medical Sciences, Swiecickiego 6 Street, 60-781 Poznan, Poland
5
Department of Orthopedics and Traumatology, Poznan University of Medical Sciences, 18 czerwca 1956r Street, 61-545 Poznan, Poland
6
Department of Pathology, Poznan University of Medical Sciences, Greater Poland Cancer Centre, Garbary 15th Street, 61-866 Poznan, Poland
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 20 November 2017 / Revised: 5 February 2018 / Accepted: 7 February 2018 / Published: 12 February 2018
(This article belongs to the Section Biochemistry)
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Abstract

Human induced pluripotent stem cells (hiPSCs) constitute an important breakthrough in regenerative medicine, particularly in orthopedics, where more effective treatments are urgently needed. Despite the promise of hiPSCs only limited data on in vitro chondrogenic differentiation of hiPSCs are available. Therefore, we compared the gene expression profile of pluripotent genes in hiPSC-derived chondrocytes (ChiPS) to that of an hiPSC cell line created by our group (GPCCi001-A). The results are shown on heatmaps and plots and confirmed by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) analysis. Unlike the ChiPS, our GPCCi001-A cells maintained their pluripotency state during long-term culture, thus demonstrating that this cell line was comprised of stable, fully pluripotent hiPSCs. Moreover, these chondrocyte-like cells not only presented features that are characteristic of chondrocytes, but they also lost their pluripotency, which is an important advantage in favor of using this cell line in future clinical studies. View Full-Text
Keywords: human induced pluripotent stem cells; chondrogenic differentiation; gene expression profile human induced pluripotent stem cells; chondrogenic differentiation; gene expression profile
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Stelcer, E.; Kulcenty, K.; Rucinski, M.; Jopek, K.; Trzeciak, T.; Richter, M.; Wroblewska, J.P.; Suchorska, W.M. Expression of Pluripotency Genes in Chondrocyte-Like Cells Differentiated from Human Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 2018, 19, 550.

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