De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome
AbstractGrasshoppers are common pests but also have high nutritional and commercial potential. Xenocatantops brachycerus Willemse (Orthoptera: Acrididae) is an economically important grasshopper species that is reared in China. Using the IlluminaHiSeqTM 4000 platform, three transcriptomes of the adult male, adult female, and nymph of X. brachycerus were sequenced. A total of 133,194,848 clean reads were obtained and de novo assembled into 43,187 unigenes with an average length of 964 bp (N50 of 1799 bp); of these, 24,717 (57.23%) unigenes matched known proteins. Based on these annotations, many putative transcripts related to X. brachycerus growth, development, environmental adaptability, and metabolism of nutritional components and bioactive components were identified. In addition, the expression profiles of all three transcriptome datasets were analyzed, and many differentially expressed genes were detected using RSEM and PossionDis. Unigenes. Unigenes with functions associated with growth and development exhibited higher transcript levels at the nymph stage, and unigenes associated with environmental adaptability showed increased transcription in the adults. These comprehensive X. brachycerus transcriptomic data will provide a useful molecular resource for gene prediction, molecular marker development, and studies on signaling pathways in this species and will serve as a reference for the efficient use of other grasshoppers. View Full-Text
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Zhao, L.; Zhang, X.; Qiu, Z.; Huang, Y. De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome. Int. J. Mol. Sci. 2018, 19, 520.
Zhao L, Zhang X, Qiu Z, Huang Y. De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome. International Journal of Molecular Sciences. 2018; 19(2):520.Chicago/Turabian Style
Zhao, Le; Zhang, Xinmei; Qiu, Zhongying; Huang, Yuan. 2018. "De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome." Int. J. Mol. Sci. 19, no. 2: 520.
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