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Endometrial Intracrinology: Oestrogens, Androgens and Endometrial Disorders
Article

Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development: The Plausible Role of miR-139-5p and miR-375

1
Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, 50090 Tartu, Estonia
2
Competence Centre on Health Technologies, 50410 Tartu, Estonia
3
Institute of Computer Science, University of Tartu, 50090 Tartu, Estonia
4
Tartu University Hospital’s Women’s Clinic, 50406 Tartu, Estonia
5
Department of Gynecology and Obstetrics, University of Münster, 48149 Münster, Germany
6
Department of Biomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, 50090 Tartu, Estonia
7
Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, Finland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(12), 3789; https://doi.org/10.3390/ijms19123789
Received: 19 October 2018 / Revised: 16 November 2018 / Accepted: 24 November 2018 / Published: 28 November 2018
(This article belongs to the Special Issue Molecular Research of Endometrial Pathophysiology)
microRNA (miRNA) expression level alterations between endometrial tissue and endometriotic lesions indicate their involvement in endometriosis pathogenesis. However, as both endometrium and endometriotic lesions consist of different cell types in various proportions, it is not clear which cells contribute to variability in miRNA levels and the overall knowledge about cell-type specific miRNA expression in ectopic cells is scarce. Therefore, we utilized fluorescence-activated cell sorting to isolate endometrial stromal cells from paired endometrial and endometrioma biopsies and combined it with high-throughput sequencing to determine miRNA alterations in endometriotic stroma. The analysis revealed 149 abnormally expressed miRNAs in endometriotic lesions, including extensive upregulation of miR-139-5p and downregulation of miR-375 compared to eutopic cells. miRNA transfection experiments in the endometrial stromal cell line ST-T1b showed that the overexpression of miR-139-5p resulted in the downregulation of homeobox A9 (HOXA9) and HOXA10 expression, whereas the endothelin 1 (EDN1) gene was regulated by miR-375. The results of this study provide further insights into the complex molecular mechanisms involved in endometriosis pathogenesis and demonstrate the necessity for cell-type-specific analysis of ectopic tissues to understand the interactions between different cell populations in disease onset and progression. View Full-Text
Keywords: endometriosis; ectopic stroma; microRNA; small RNA sequencing; EDN1; HOXA10; miR-139-5p; miR-375 endometriosis; ectopic stroma; microRNA; small RNA sequencing; EDN1; HOXA10; miR-139-5p; miR-375
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MDPI and ACS Style

Rekker, K.; Tasa, T.; Saare, M.; Samuel, K.; Kadastik, Ü.; Karro, H.; Götte, M.; Salumets, A.; Peters, M. Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development: The Plausible Role of miR-139-5p and miR-375. Int. J. Mol. Sci. 2018, 19, 3789. https://doi.org/10.3390/ijms19123789

AMA Style

Rekker K, Tasa T, Saare M, Samuel K, Kadastik Ü, Karro H, Götte M, Salumets A, Peters M. Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development: The Plausible Role of miR-139-5p and miR-375. International Journal of Molecular Sciences. 2018; 19(12):3789. https://doi.org/10.3390/ijms19123789

Chicago/Turabian Style

Rekker, Kadri; Tasa, Tõnis; Saare, Merli; Samuel, Külli; Kadastik, Ülle; Karro, Helle; Götte, Martin; Salumets, Andres; Peters, Maire. 2018. "Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development: The Plausible Role of miR-139-5p and miR-375" Int. J. Mol. Sci. 19, no. 12: 3789. https://doi.org/10.3390/ijms19123789

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