Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [Cl−]i Decrease in Ciliated Human Nasal Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. Video Images of cHNECs Activated by Daidzein
2.2. Effects of Daidzein on CBD and CBF
2.3. Effects of Daidzein on [Cl−]i, CBD, and CBF
2.4. Latex Microbeads Movement Driven by the Beating Cilia of cHNECs
2.5. Effects of Ca2+ and cAMP on CBD and CBF Stimulated by Daidzein
2.6. Effects of Daidzein on CBD and CBF at 25 °C
3. Discussion
4. Materials and Methods
4.1. Ethical Approval
4.2. Solution and Chemicals
4.3. Cell Preparation and Culture
4.4. CBD and CBF Measurements
4.5. Measurement of [Cl−]i
4.6. Observation of Latex Microbead Transport in cHNECs
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Inui, T.-a.; Yasuda, M.; Hirano, S.; Ikeuchi, Y.; Kogiso, H.; Inui, T.; Marunaka, Y.; Nakahari, T. Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [Cl−]i Decrease in Ciliated Human Nasal Epithelial Cells. Int. J. Mol. Sci. 2018, 19, 3754. https://doi.org/10.3390/ijms19123754
Inui T-a, Yasuda M, Hirano S, Ikeuchi Y, Kogiso H, Inui T, Marunaka Y, Nakahari T. Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [Cl−]i Decrease in Ciliated Human Nasal Epithelial Cells. International Journal of Molecular Sciences. 2018; 19(12):3754. https://doi.org/10.3390/ijms19123754
Chicago/Turabian StyleInui, Taka-aki, Makoto Yasuda, Shigeru Hirano, Yukiko Ikeuchi, Haruka Kogiso, Toshio Inui, Yoshinori Marunaka, and Takashi Nakahari. 2018. "Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [Cl−]i Decrease in Ciliated Human Nasal Epithelial Cells" International Journal of Molecular Sciences 19, no. 12: 3754. https://doi.org/10.3390/ijms19123754