Next Article in Journal
Ageing, Cellular Senescence and Neurodegenerative Disease
Next Article in Special Issue
Dysregulated Marginal Zone B Cell Compartment in a Mouse Model of Sjögren’s Syndrome with Ocular Inflammation
Previous Article in Journal
Galectins in Cancer and Translational Medicine: From Bench to Bedside
Previous Article in Special Issue
Severity of Sjögren’s Syndrome Keratoconjunctivitis Sicca Increases with Increased Percentage of Conjunctival Antigen-Presenting Cells
Open AccessArticle

Immune Response Targeting Sjögren’s Syndrome Antigen Ro52 Suppresses Tear Production in Female Mice

Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(10), 2935; https://doi.org/10.3390/ijms19102935
Received: 31 August 2018 / Revised: 23 September 2018 / Accepted: 24 September 2018 / Published: 27 September 2018
(This article belongs to the Special Issue Mechanisms of Disease in Sjögren Syndrome)
Autoantibodies reactive against Ro52 are present in 70% of Sjögren’s syndrome patients and are associated with higher disease severity. However, their role in causing aqueous deficient dry eye, a major cause for morbidity in Sjögren’s syndrome, is unclear. To investigate whether immune responses targeting Ro52 contribute towards the dry eye, male and female NZM2758 mice were immunized with recombinant Ro52. Tear production was measured by the phenol red thread test. Sera were analyzed for anti-Ro52 levels by immunoprecipitation. Lacrimal glands were evaluated for inflammatory foci and IgG deposits. Our results showed that, although all mice generated anti-Ro52 antibodies, only females developed a significant drop in tear production. None of the mice developed severe lacrimal gland inflammation, and female mice with anti-Ro52 showed higher levels of IgG deposits within their glands. Passive transfer of anti-Ro52 sera caused reduced tear production in female mice, but not in males. This study demonstrates for the first time that immune responses initiated by Ro52 induce aqueous dry eye, and this may be driven by anti-Ro52 antibodies. Furthermore, the sexual dimorphism in glandular dysfunction suggests that the lacrimal glands in females are more susceptible to autoantibody-mediated injury. View Full-Text
Keywords: Sjögren’s syndrome; Ro52; lacrimal gland; dry eye; sexual dimorphism Sjögren’s syndrome; Ro52; lacrimal gland; dry eye; sexual dimorphism
Show Figures

Figure 1

MDPI and ACS Style

Trzeciak, M.; Bagavant, H.; Papinska, J.; Deshmukh, U.S. Immune Response Targeting Sjögren’s Syndrome Antigen Ro52 Suppresses Tear Production in Female Mice. Int. J. Mol. Sci. 2018, 19, 2935. https://doi.org/10.3390/ijms19102935

AMA Style

Trzeciak M, Bagavant H, Papinska J, Deshmukh US. Immune Response Targeting Sjögren’s Syndrome Antigen Ro52 Suppresses Tear Production in Female Mice. International Journal of Molecular Sciences. 2018; 19(10):2935. https://doi.org/10.3390/ijms19102935

Chicago/Turabian Style

Trzeciak, Marta; Bagavant, Harini; Papinska, Joanna; Deshmukh, Umesh S. 2018. "Immune Response Targeting Sjögren’s Syndrome Antigen Ro52 Suppresses Tear Production in Female Mice" Int. J. Mol. Sci. 19, no. 10: 2935. https://doi.org/10.3390/ijms19102935

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop