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Seed Endophyte Microbiome of Crotalaria pumila Unpeeled: Identification of Plant-Beneficial Methylobacteria

Centre for Environmental Sciences, Hasselt University, Agoralaan building D, 3590 Diepenbeek, Belgium
Laboratory of Environmental Chemistry and Environmental Microbiology, Edaphology, Colegio de Postgraduados, Campus Montecillo, Carretera Mexico-Texcoco km 36.5, Montecillo 56230, Mexico
Laboratory of Cell Biology and Histology, University of Antwerp Campus Drie Eiken, Universiteitsplein 1, 2610 Wilrijk, Antwerp, Belgium
Department of Biology, Thompson Rivers University, 950 McGill Road, Kamloops, BC V2C0E1, Canada
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(1), 291;
Received: 15 December 2017 / Revised: 13 January 2018 / Accepted: 15 January 2018 / Published: 19 January 2018
(This article belongs to the Special Issue Plant Microbe Interaction 2017)
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Metal contaminated soils are increasing worldwide. Metal-tolerant plants growing on metalliferous soils are fascinating genetic and microbial resources. Seeds can vertically transmit endophytic microorganisms that can assist next generations to cope with environmental stresses, through yet poorly understood mechanisms. The aims of this study were to identify the core seed endophyte microbiome of the pioneer metallophyte Crotalaria pumila throughout three generations, and to better understand the plant colonisation of the seed endophyte Methylobacterium sp. Cp3. Strain Cp3 was detected in C. pumila seeds across three successive generations and showed the most dominant community member. When inoculated in the soil at the time of flowering, strain Cp3 migrated from soil to seeds. Using confocal microscopy, Cp3-mCherry was demonstrated to colonise the root cortex cells and xylem vessels of the stem under metal stress. Moreover, strain Cp3 showed genetic and in planta potential to promote seed germination and seedling development. We revealed, for the first time, that the seed microbiome of a pioneer plant growing in its natural environment, and the colonisation behaviour of an important plant growth promoting systemic seed endophyte. Future characterization of seed microbiota will lead to a better understanding of their functional contribution and the potential use for seed-fortification applications. View Full-Text
Keywords: metalliferous soil; trace metals; Methylobacterium; seed core microbiome; plant growth-promoting endophyte; xylem metalliferous soil; trace metals; Methylobacterium; seed core microbiome; plant growth-promoting endophyte; xylem

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sánchez-López, A.S.; Pintelon, I.; Stevens, V.; Imperato, V.; Timmermans, J.-P.; González-Chávez, C.; Carrillo-González, R.; Van Hamme, J.; Vangronsveld, J.; Thijs, S. Seed Endophyte Microbiome of Crotalaria pumila Unpeeled: Identification of Plant-Beneficial Methylobacteria. Int. J. Mol. Sci. 2018, 19, 291.

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