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Int. J. Mol. Sci. 2018, 19(1), 124; https://doi.org/10.3390/ijms19010124

Extracorporeal Shock Wave Therapy Alters the Expression of Fibrosis-Related Molecules in Fibroblast Derived from Human Hypertrophic Scar

1
Burn Institute, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, Korea
2
Department of Pediatrics, Hallym University Hangang Sacred Heart Hospital, Seoul 07247, Korea
3
Department of Rehabilitation Medicine, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 31 October 2017 / Revised: 22 December 2017 / Accepted: 26 December 2017 / Published: 2 January 2018
(This article belongs to the Special Issue Recent Advances in Scar Biology)
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Abstract

Extracorporeal shock wave therapy (ESWT) considerably improves the appearance and symptoms of post-burn hypertrophic scars (HTS). However, the mechanism underlying the observed beneficial effects is not well understood. The objective of this study was to elucidate the mechanism underlying changes in cellular and molecular biology that is induced by ESWT of fibroblasts derived from scar tissue (HTSFs). We cultured primary dermal fibroblasts derived from human HTS and exposed these cells to 1000 impulses of 0.03, 0.1, and 0.3 mJ/mm2. At 24 h and 72 h after treatment, real-time PCR and western blotting were used to detect mRNA and protein expression, respectively, and cell viability and mobility were assessed. While HTSF viability was not affected, migration was decreased by ESWT. Transforming growth factor beta 1 (TGF-β1) expression was reduced and alpha smooth muscle actin (α-SMA), collagen-I, fibronectin, and twist-1 were reduced significantly after ESWT. Expression of E-cadherin was increased, while that of N-cadherin was reduced. Expression of inhibitor of DNA binding 1 and 2 was increased. In conclusion, suppressed epithelial-mesenchymal transition might be responsible for the anti-scarring effect of ESWT, and has potential as a therapeutic target in the management of post-burn scars. View Full-Text
Keywords: extracorporeal shock wave therapy; burn hypertrophic scar; hypertrophic scar-derived fibroblast; epithelial-mesenchymal transition; inhibitor of DNA binding protein extracorporeal shock wave therapy; burn hypertrophic scar; hypertrophic scar-derived fibroblast; epithelial-mesenchymal transition; inhibitor of DNA binding protein
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Cui, H.S.; Hong, A.R.; Kim, J.-B.; Yu, J.H.; Cho, Y.S.; Joo, S.Y.; Seo, C.H. Extracorporeal Shock Wave Therapy Alters the Expression of Fibrosis-Related Molecules in Fibroblast Derived from Human Hypertrophic Scar. Int. J. Mol. Sci. 2018, 19, 124.

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