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Int. J. Mol. Sci. 2017, 18(8), 1731;

Serum Aberrant N-Glycan Profile as a Marker Associated with Early Antibody-Mediated Rejection in Patients Receiving a Living Donor Kidney Transplant

Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan
Department of Advanced Transplant and Regenerative Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan
Graduate School of Life Science, Frontier Research Center for Advanced Material and Life Science, Hokkaido University, Sapporo 060-0810, Japan
Department of Urology, St. Marianna University of Medicine, Kawasaki 216-8511, Japan
Department of Urology, Akita University Graduate School of Medicine, Akita 010-8543, Japan
Department of Kidney Transplant Surgery, Sapporo City General Hospital, Sapporo 060-8611, Japan
Department of Urology, Tokyo-Woman’s Medical University, Tokyo 162-8666, Japan
Author to whom correspondence should be addressed.
Received: 6 June 2017 / Revised: 1 August 2017 / Accepted: 7 August 2017 / Published: 8 August 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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We determined if the serum N-glycan profile can be used as a diagnostic marker of antibody-mediated rejection (ABMR) in living donor kidney transplant (LKTx) recipients. Glycoblotting, combined with mass spectrometry, was used to retrospectively examine N-glycan levels in the postoperative sera of 197 LKTx recipients of whom 16 recipients had ABMR with or without T-cell-mediated rejection (TCMR), 40 recipients had TCMR, and 141 recipients had no adverse events. Multivariate discriminant analysis for prediction of ABMR was performed by inputting an ABMR event as an explanatory variable and sex, age, and serum N-glycan level as objective variables. The N-glycan score was calculated by multiplying the level of candidate objective variables by objective function values. The ABMR predictive performance of the N-glycan score was assessed by receiver operator characteristic curve and Kaplan–Meier curve analyses. The N-glycan score discriminated ABMR with 81.25% sensitivity, 87.85% specificity, and an area under the curve (AUC) of 0.892 that was far superior to that of preformed donor-specific antibody status (AUC, 0.761). Recipients with N-glycan-positive scores >0.8770 had significantly shorter ABMR survival than that of recipients with N-glycan-negative scores. Although the limitations of our study includ its small sample size and retrospective nature, the serum N-glycan score may contribute to prediction of ABMR. View Full-Text
Keywords: biomarker; antibody-mediated rejection; N-glycan biomarker; antibody-mediated rejection; N-glycan

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Noro, D.; Yoneyama, T.; Hatakeyama, S.; Tobisawa, Y.; Mori, K.; Hashimoto, Y.; Koie, T.; Tanaka, M.; Nishimura, S.-I.; Sasaki, H.; Saito, M.; Harada, H.; Chikaraishi, T.; Ishida, H.; Tanabe, K.; Satoh, S.; Ohyama, C. Serum Aberrant N-Glycan Profile as a Marker Associated with Early Antibody-Mediated Rejection in Patients Receiving a Living Donor Kidney Transplant. Int. J. Mol. Sci. 2017, 18, 1731.

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