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Application of Extrusion-Based Hydrogel Bioprinting for Cartilage Tissue Engineering
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Int. J. Mol. Sci. 2017, 18(8), 1707;

Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel

Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 200-702, Korea
Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon 200-704, Korea
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 3 July 2017 / Revised: 29 July 2017 / Accepted: 1 August 2017 / Published: 4 August 2017
(This article belongs to the Special Issue Three-dimensional (3D) Bioprinting of Tissues and Organs)
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Several methods for auricular cartilage engineering use tissue engineering techniques. However, an ideal method for engineering auricular cartilage has not been reported. To address this issue, we developed a strategy to engineer auricular cartilage using silk fibroin (SF) and polyvinyl alcohol (PVA) hydrogel. We constructed different hydrogels with various ratios of SF and PVA by using salt leaching, silicone mold casting, and freeze-thawing methods. We characterized each of the hydrogels in terms of the swelling ratio, tensile strength, pore size, thermal properties, morphologies, and chemical properties. Based on the cell viability results, we found a blended hydrogel composed of 50% PVA and 50% SF (P50/S50) to be the best hydrogel among the fabricated hydrogels. An intact 3D ear-shaped auricular cartilage formed six weeks after the subcutaneous implantation of a chondrocyte-seeded 3D ear-shaped P50/S50 hydrogel in rats. We observed mature cartilage with a typical lacunar structure both in vitro and in vivo via histological analysis. This study may have potential applications in auricular tissue engineering with a human ear-shaped hydrogel. View Full-Text
Keywords: silk fibroin; 3D printer; auricular cartilage silk fibroin; 3D printer; auricular cartilage

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lee, J.M.; Sultan, M.T.; Kim, S.H.; Kumar, V.; Yeon, Y.K.; Lee, O.J.; Park, C.H. Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel. Int. J. Mol. Sci. 2017, 18, 1707.

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