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The Impact of Melatonin on Colon Cancer Cells’ Resistance to Doxorubicin in an in Vitro Study

Department of Histology and Embryology, Wroclaw Medical University, Wroclaw 50-368, Poland
Department of Histology and Embryology, Poznan Medical University, Wroclaw 60-781, Poland
Department of Physiotherapy, University School of Physical Education, Wroclaw 51-612, Poland
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(7), 1396;
Received: 31 March 2017 / Revised: 18 May 2017 / Accepted: 23 June 2017 / Published: 29 June 2017
(This article belongs to the Special Issue Melatonin and Its Analogues: Experimental and Clinical Aspects)
Multi-drug resistance (MDR) is the main cause of low effectiveness of cancer chemotherapy. P-glycoprotein (P-gp) is one of the main factors determining MDR. Some studies indicate the potential role of melatonin (MLT) in MDR. In this study, we examined the effect of MLT on colon cancer cell’s resistance to doxorubicin (DOX). Using the sulforhodamine B (SRB), method the effect of tested substances on the survival of LoVo (colon cancer cells sensitive to DOX) and LoVoDX (colon cancer cells resistant to DOX) was rated. Using immunocytochemistry (ICC), the expression of P-gp in the LoVo and LoVoDX was determined. With the real-time PCR (RT-PCR) technique, the ABCB1 expression in LoVoDX was evaluated. Based on the results, it was found that MLT in some concentrations intensified the cytotoxicity effect of DOX in the LoVoDX cells. In the ICC studies, it was demonstrated that certain concentrations of MLT and DOX cause an increase in the percentage of cells expressing P-gp, which correlates positively with ABCB1 expression (RT-PCR). The mechanism of overcoming resistance by MLT is probably not only associated with the expression of P-gp. It seems appropriate to carry out further research on the use of MLT as the substance supporting cancer chemotherapy. View Full-Text
Keywords: melatonin; P-glycoprotein; multi drug resistance; doxorubicin melatonin; P-glycoprotein; multi drug resistance; doxorubicin
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Fic, M.; Gomulkiewicz, A.; Grzegrzolka, J.; Podhorska-Okolow, M.; Zabel, M.; Dziegiel, P.; Jablonska, K. The Impact of Melatonin on Colon Cancer Cells’ Resistance to Doxorubicin in an in Vitro Study. Int. J. Mol. Sci. 2017, 18, 1396.

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