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Article

Design and Synthesis of Non-Peptide Mimetics Mapping the Immunodominant Myelin Basic Protein (MBP83–96) Epitope to Function as T-Cell Receptor Antagonists

1
Department of Chemistry, University of Patras, 26504 Rion Patras, Greece
2
School of Chemistry, Cardiff University, Park Place, Cardiff CF103AT, Wales, UK
3
Laboratory of Immunohematology, Division of Hematology, Department of Internal Medicine, Medical School, University of Patras, Rion, 26500 Patras, Greece
4
Centre for Chronic Disease, College of Health and Biomedicine, Victoria University, St. Albans, VIC 3021, Australia
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Christoph Kleinschnitz
Int. J. Mol. Sci. 2017, 18(6), 1215; https://doi.org/10.3390/ijms18061215
Received: 26 April 2017 / Revised: 2 June 2017 / Accepted: 2 June 2017 / Published: 8 June 2017
(This article belongs to the Special Issue Advances in Multiple Sclerosis 2017)
Encephalitogenic T cells are heavily implicated in the pathogenesis of multiple sclerosis (MS), an autoimmune demyelinating disease of the central nervous system. Their stimulation is triggered by the formation of a trimolecular complex between the human leukocyte antigen (HLA), an immunodominant myelin basic protein (MBP) epitope, and the T cell receptor (TCR). We detail herein our studies directed towards the rational design and synthesis of non-peptide mimetic molecules, based on the immunodominant MBP83–96 epitope that is recognized by the TCR in complex with HLA. We focused our attention on the inhibition of the trimolecular complex formation and consequently the inhibition of proliferation of activated T cells. A structure-based pharmacophore model was generated, in view of the interactions between the TCR and the HLA-MBP83–96 complex. As a result, new candidate molecules were designed based on lead compounds obtained through the ZINC database. Moreover, semi-empirical and density functional theory methods were applied for the prediction of the binding energy between the proposed non-peptide mimetics and the TCR. We synthesized six molecules that were further evaluated in vitro as TCR antagonists. Analogues 15 and 16 were able to inhibit to some extent the stimulation of T cells by the immunodominant MBP83–99 peptide from immunized mice. Inhibition was followed to a lesser degree by analogues 17 and 18 and then by analogue 19. These studies show that lead compounds 15 and 16 may be used for immunotherapy against MS. View Full-Text
Keywords: multiple sclerosis; trimolecular complex; rational drug design; non-peptide mimetics; molecular modeling; cell proliferation; T cell antagonism multiple sclerosis; trimolecular complex; rational drug design; non-peptide mimetics; molecular modeling; cell proliferation; T cell antagonism
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MDPI and ACS Style

Yannakakis, M.-P.; Simal, C.; Tzoupis, H.; Rodi, M.; Dargahi, N.; Prakash, M.; Mouzaki, A.; Platts, J.A.; Apostolopoulos, V.; Tselios, T.V. Design and Synthesis of Non-Peptide Mimetics Mapping the Immunodominant Myelin Basic Protein (MBP83–96) Epitope to Function as T-Cell Receptor Antagonists. Int. J. Mol. Sci. 2017, 18, 1215. https://doi.org/10.3390/ijms18061215

AMA Style

Yannakakis M-P, Simal C, Tzoupis H, Rodi M, Dargahi N, Prakash M, Mouzaki A, Platts JA, Apostolopoulos V, Tselios TV. Design and Synthesis of Non-Peptide Mimetics Mapping the Immunodominant Myelin Basic Protein (MBP83–96) Epitope to Function as T-Cell Receptor Antagonists. International Journal of Molecular Sciences. 2017; 18(6):1215. https://doi.org/10.3390/ijms18061215

Chicago/Turabian Style

Yannakakis, Mary-Patricia, Carmen Simal, Haralambos Tzoupis, Maria Rodi, Narges Dargahi, Monica Prakash, Athanasia Mouzaki, James A. Platts, Vasso Apostolopoulos, and Theodore V. Tselios. 2017. "Design and Synthesis of Non-Peptide Mimetics Mapping the Immunodominant Myelin Basic Protein (MBP83–96) Epitope to Function as T-Cell Receptor Antagonists" International Journal of Molecular Sciences 18, no. 6: 1215. https://doi.org/10.3390/ijms18061215

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