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Open AccessArticle

Characterization of Copy Number Variation’s Potential Role in Marek’s Disease

1
Department of Animal & Avian Sciences, University of Maryland, College Park, MD 20742, USA
2
Animal Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA
3
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100864, China
4
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource, No. 95 Wenhua Road, Zhengzhou 450002, China
5
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China
6
Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt
7
USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Klaus Wimmers
Int. J. Mol. Sci. 2017, 18(5), 1020; https://doi.org/10.3390/ijms18051020
Received: 27 March 2017 / Revised: 22 April 2017 / Accepted: 4 May 2017 / Published: 9 May 2017
(This article belongs to the Special Issue Exploring the Genotype–Phenotype Map to Explain Complex Traits)
Marek’s Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72, as well as their recombinant congenic strains (RCS) with varied susceptibility to MD, are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we investigated copy number variation (CNV) in these inbred chicken lines using the Affymetrix Axiom HD 600 K SNP genotyping array. We detected 393 CNV segments across all ten chicken lines, of which 12 CNVs were specifically identified in Line 72. We then assessed genetic structure based on CNV and observed markedly different patterns. Finally, we validated two deletion events in Line 72 and correlated them with genes expression using qPCR and RNA-seq, respectively. Our combined results indicated that these two CNV deletions were likely to contribute to MD susceptibility. View Full-Text
Keywords: copy number variation; chicken; inbred lines; recombinant congenic strains (RCS); Marek’s disease; RNA-sequencing copy number variation; chicken; inbred lines; recombinant congenic strains (RCS); Marek’s disease; RNA-sequencing
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MDPI and ACS Style

Xu, L.; He, Y.; Ding, Y.; Sun, G.; Carrillo, J.A.; Li, Y.; Ghaly, M.M.; Ma, L.; Zhang, H.; Liu, G.E.; Song, J. Characterization of Copy Number Variation’s Potential Role in Marek’s Disease. Int. J. Mol. Sci. 2017, 18, 1020.

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