TβRII Regulates the Proliferation of Metanephric Mesenchyme Cells through Six2 In Vitro
Abstract
:1. Introduction
2. Results
2.1. TβRII Promoted the Proliferation Rate of Metanephric Mesenchyme (MM) Cells
2.2. TβRII Increased the Expression of Six2
2.3. Overexpression of Six2 Can Rescue the Proliferation of MM Cells Induced by TβRII Deficiency
2.4. Smad3 Can Transcriptionally Regulate Six2 and Partially Rescue the Proliferation Phenotype
3. Discussions
4. Materials and Methods
4.1. Cell Culture and Transfection
4.2. Plasmid Construction
4.3. Luciferase Reporter Assay
4.4. Collection of Embryo Kidney
4.5. Real-time Polymerase Chain Reaction (PCR)
4.6. Western Blot
4.7. Drug Stimulation
4.8. Immunofluorescence
4.9. EdU Cell Proliferation Assay
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Mao, Z.; Lyu, Z.; Huang, L.; Zhou, Q.; Weng, Y. TβRII Regulates the Proliferation of Metanephric Mesenchyme Cells through Six2 In Vitro. Int. J. Mol. Sci. 2017, 18, 853. https://doi.org/10.3390/ijms18040853
Mao Z, Lyu Z, Huang L, Zhou Q, Weng Y. TβRII Regulates the Proliferation of Metanephric Mesenchyme Cells through Six2 In Vitro. International Journal of Molecular Sciences. 2017; 18(4):853. https://doi.org/10.3390/ijms18040853
Chicago/Turabian StyleMao, Zhaomin, Zhongshi Lyu, Liyuan Huang, Qin Zhou, and Yaguang Weng. 2017. "TβRII Regulates the Proliferation of Metanephric Mesenchyme Cells through Six2 In Vitro" International Journal of Molecular Sciences 18, no. 4: 853. https://doi.org/10.3390/ijms18040853