Chemokine CXCL7 Heterodimers: Structural Insights, CXCR2 Receptor Function, and Glycosaminoglycan Interactions
Abstract
:1. Introduction
2. Results
2.1. NMR (Nuclear Magnetic Resonance) Characterization of CXCL7 Heterodimers
2.2. Molecular Dynamics of Chemokine Heterodimers
2.3. Design and Characterization of a Trapped Heterodimer
2.4. Heterodimer-GAG Interactions
2.5. Heterodimer Receptor Binding Activity
3. Discussion
4. Materials and Methods
4.1. Molecular Dynamics Simulations
4.2. Expression and Purification of Chemokines
4.3. NMR Spectroscopy
4.4. Heparin-Heterodimer Interactions
4.5. Heterodimer-GAG Docking
4.6. Receptor Activity of the Heterodimer
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
CXCL | CXC ligand |
CXCR2 | CXC chemokine receptor 2 |
CXCL7/NAP2 | Neutrophil-activating peptide 2 |
GAG | Glycosaminoglycan |
NAC | CXCR2 N-terminal domain |
NMR | Nuclear Magnetic Resonance |
HSQC | Heteronuclear single quantum coherence |
CSP | Chemical shift perturbation |
NOE | Nuclear Overhauser effect |
AIR | Ambiguous interaction restraints |
Dp8 | Heparin octasaccharide |
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Brown, A.J.; Joseph, P.R.B.; Sawant, K.V.; Rajarathnam, K. Chemokine CXCL7 Heterodimers: Structural Insights, CXCR2 Receptor Function, and Glycosaminoglycan Interactions. Int. J. Mol. Sci. 2017, 18, 748. https://doi.org/10.3390/ijms18040748
Brown AJ, Joseph PRB, Sawant KV, Rajarathnam K. Chemokine CXCL7 Heterodimers: Structural Insights, CXCR2 Receptor Function, and Glycosaminoglycan Interactions. International Journal of Molecular Sciences. 2017; 18(4):748. https://doi.org/10.3390/ijms18040748
Chicago/Turabian StyleBrown, Aaron J., Prem Raj B. Joseph, Kirti V. Sawant, and Krishna Rajarathnam. 2017. "Chemokine CXCL7 Heterodimers: Structural Insights, CXCR2 Receptor Function, and Glycosaminoglycan Interactions" International Journal of Molecular Sciences 18, no. 4: 748. https://doi.org/10.3390/ijms18040748