The Role of the Melatoninergic System in Light-Entrained Behavior of Mice
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Animals
- (1)
- (2)
- (3)
- C3H/HeN-Pde6brd1−,−/mt1−,−/mt2−,−/mt1,2−,− (short: C3H MT 1 KO, n = 6/C3H MT 2 KO, n = 6/C3H MT 1,2 KO, n = 6). Mice with a targeted deletion of the MT1 gene (C3H MT1 KO, [30]) and the MT2 gene (C3H MT2 KO, [31]) were bred on a melatonin-proficient C3H/HeN-Pde6brd1−,− background for at least 10 generations. These single KO mice were kindly provided by Dr. David Weaver (UMASS Medical School, Worcester, MA, USA). C3H MT1,2 KO double deficient mice were obtained in our lab by crossing C3H MT1 KO and C3H MT2 KO mice and breeding the MT1/2 double KO offspring for at least 10 generations [17].
4.2. Animal Housing, Entrainment and Data Recording
4.2.1. Housing and Entrainment
4.2.2. Locomotor Activity/Actograms
4.3. Measurement Parameters Derived from the Actograms
4.4. Experimental Design and Statistics
Acknowledgments
Author Contributions
Conflicts of Interest
References
- Arendt, J. Shift work: Coping with the biological clock. Occup. Med. 2010, 60, 10–20. [Google Scholar] [CrossRef] [PubMed]
- Cho, K. Chronic “jet lag” produces temporal lobe atrophy and spatial cognitive deficits. Nat. Neurosci. 2001, 4, 567–568. [Google Scholar] [CrossRef] [PubMed]
- Touitou, Y. Adolescent sleep misalignment: A chronic jet lag and a matter of public health. J. Physiol. 2013, 107, 323–326. [Google Scholar] [CrossRef] [PubMed]
- Davidson, A.J.; Sellix, M.T.; Daniel, J.; Yamazaki, S.M.; Menaker, M.; Block, G.D. Chronic jet lag increases mortality in aged mice. Curr. Biol. 2006, 16, 914–916. [Google Scholar] [CrossRef] [PubMed]
- Filipski, E.; Delaunay, F.; King, V.M.; Wu, M.W.; Claustrat, B.; Gréchez-Cassiau, A.; Guettier, C.; Hastings, M.H.; Francis, L. Effects of chronic jet lag on tumor progression in mice. Cancer Res. 2004, 64, 7879–7885. [Google Scholar] [CrossRef] [PubMed]
- Turek, F.W.; Penev, P.; Zhang, Y.; van Reeth, O.; Zee, P. Effects of age on the circadian system. Neurosci. Biobehav. Rev. 1995, 19, 53–58. [Google Scholar] [CrossRef]
- Wittmann, M.; Dinich, J.; Merrow, M.; Roenneberg, T. Social jet lag: Misalignment of biological and social time. Chronobiol. Int. 2006, 23, 497–509. [Google Scholar] [CrossRef] [PubMed]
- Chepesiuk, R. Missing the dark: Health effects of light pollution. Environ. Health Perspect. 2009, 117, A20–A27. [Google Scholar] [CrossRef] [PubMed]
- Pfeffer, M.; Korf, H.W.; von Gall, C. Chronotype and stability of spontaneous locomotor activity rhythm in BMAL1-deficient mice. Chronobiol. Int. 2015, 32, 81–91. [Google Scholar] [CrossRef] [PubMed]
- Pfeffer, M.; Wicht, H.; von Gall, C.; Korf, H.W. Owls and larks in mice. Front. Neurol. 2015, 6, 101. [Google Scholar] [CrossRef] [PubMed]
- Dijk, D.J.; Duffy, J.F.; Riel, E.; Shanahan, T.L.; Czeisler, C.A. Ageing and the circadian and homeostatic regulation of human sleep during forced desynchrony of rest, melatonin and temperature rhythms. J. Physiol. 1999, 516, 611–627. [Google Scholar] [CrossRef] [PubMed]
- Weinert, D.; Waterhouse, J. The circadian rhythm of core temperature: Effects of physical activity and aging. Physiol. Behav. 2006, 90, 246–256. [Google Scholar] [CrossRef] [PubMed]
- Farajnia, S.; Michel, S.; Deboer, T.; vander Leest, H.T.; Houben, T.; Rohling, J.H.; Ramkisoensing, A.; Yasenkov, R.; Meijer, J.H. Evidence for neuronal desynchrony in the aged suprachiasmatic nucleus clock. J. Neurosci. 2012, 32, 5891–5899. [Google Scholar] [CrossRef] [PubMed]
- Korf, H.W.; von Gall, C. Mice, melatonin and the circadian system. Mol. Cell. Endocrinol. 2006, 252, 57–68. [Google Scholar] [CrossRef] [PubMed]
- Spadoni, G.; Bedini, A.; Rivara, S.; Mor, M. Melatonin receptor agonists: New options for insomnia and depression treatment. CNS Neurosci. Ther. 2011, 17, 733–741. [Google Scholar] [CrossRef] [PubMed]
- Arendt, J.; Skene, D.J.; Middleton, B.; Lockley, S.W.; Deacon, S. Efficacy of melatonin treatment in jet lag, shift work, and blindness. J. Biol. Rhythms 1997, 12, 604–617. [Google Scholar] [CrossRef] [PubMed]
- Pfeffer, M.; Rauch, A.; Korf, H.W.; von Gall, C. The endogenous melatonin (MT) signal facilitates reentrainment of the circadian system to light-induced phase advances by acting upon MT2 receptors. Chronobiol. Int. 2012, 29, 415–429. [Google Scholar] [CrossRef] [PubMed]
- Goto, M.; Oshima, I.; Tomita, T.; Ebihara, S. Melatonin content of the pineal gland in different mouse strains. J. Pineal Res. 1989, 7, 195–204. [Google Scholar] [CrossRef] [PubMed]
- Von Gall, C.; Lewy, A.; Schomerus, C.; Vivien-Roels, B.; Pevét, P.; Korf, H.W.; Stehle, J.H. Transcription factor dynamics and neuroendocrine signalling in the mouse pineal gland: A comparative analysis of melatonin deficient C57BL mice and melatonin-proficient C3H mice. Eur. J. Neurosci. 2000, 12, 964–972. [Google Scholar] [CrossRef] [PubMed]
- Kasahara, T.; Abe, K.; Mekada, K.; Yoshiki, A.; Kato, T. Genetic variation of melatonin productivity in laboratory mice under domestication. Proc. Natl. Acad. Sci. USA 2010, 107, 6412–6417. [Google Scholar] [CrossRef] [PubMed]
- Wicht, H.; Korf, H.W.; Ackermann, H.; Ekhart, D.; Fischer, C.; Pfeffer, M. Chronotypes and rhythm stability in mice. Chronobiol. Int. 2014, 1, 27–36. [Google Scholar] [CrossRef] [PubMed]
- Dubocovich, M.L.; Hudson, R.L.; Sumaya, I.C.; Masana, M.I.; Manna, E. Effect of MT1 melatonin receptor deletion on melatonin-mediated phase shift of circadian rhythms in the C57BL/6 mouse. J. Pineal Res. 2005, 39, 113–120. [Google Scholar] [CrossRef] [PubMed]
- Lipton, J.; Megerian, J.T.; Kothare, S.V.; Cho, Y.J.; Shanahan, T.; Chart, H.; Ferber, R.; Adler-Golden, L.; Cohen, L.E.; Czeisler, C.A.; et al. Melatonin deficiency and disrupted circadian rhythms in pediatric survivors of craniopharyngioma. Neurology 2009, 73, 323–325. [Google Scholar] [CrossRef] [PubMed]
- Slominski, R.M.; Reiter, R.J.; Schlabritz-Loutsevitch, N.; Ostrom, R.S.; Slominski, A.T. Melatonin membrane receptors in peripheral tissues: Distribution and functions. Mol. Cell. Endocrinol. 2012, 351, 152–166. [Google Scholar] [CrossRef] [PubMed]
- Lacoste, B.; Angeloni, D.; Dominguez-Lopez, S.; Calderoni, S.; Mauro, A.; Fraschini, F.; Descarries, L.; Gobbi, G. Anatomical and cellular localization of melatonin MT1 and MT2 receptors in the adult rat brain. J. Pineal Res. 2015, 58, 397–417. [Google Scholar] [CrossRef] [PubMed]
- Storer, J.B. Longevity and gross pathology at death in 22 inbred mouse strains. J. Gerontol. 1966, 2, 404–409. [Google Scholar] [CrossRef]
- Daan, S.; Spoelstra, K.; Albrecht, U.; Schmutz, I.; Daan, M.; Daan, B.; Rienks, F.; Poletaeva, I.; dell’Omo, G.; Vyssotski, A.; et al. Lab mice in the field: Unorthodox daily activity and effects of a dysfunctional circadian clock allele. J. Biol. Rhythms 2011, 26, 118–129. [Google Scholar] [CrossRef] [PubMed]
- Pittendrigh, C.S.; Daan, S. A functional analysis of circadian pacemakers in nocturnal rodents. J. Comp. Physiol. 1976, 106, 223–252. [Google Scholar] [CrossRef]
- Refinetti, R. Non-stationary time series and the robustness of circadian rhythms. J. Theor. Biol. 2004, 227, 571–581. [Google Scholar] [CrossRef] [PubMed]
- Liu, C.; Weaver, D.R.; Jin, X.; Shearman, L.P.; Pieschl, R.L.; Gribkoff, V.K.; Reppert, S.M. Molecular dissection of two distinct actions of melatonin on the suprachiasmatic circadian clock. Neuron 1997, 19, 91–102. [Google Scholar] [CrossRef]
- Jin, X.; Shearman, L.P.; Weaver, D.R.; Zylka, M.J.; de Vries, G.J.; Reppert, S.M. A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock. Cell 1999, 96, 57–68. [Google Scholar] [CrossRef]
- Ackermann, H. BIAS. A program package for biometrical analysis of samples. Comp. Stat. Dat. Anal. 1999, 11, 223–224. [Google Scholar] [CrossRef]
Measurement over 10–12 Days. Interstrain Comparisons of mean MoA and General Stability via Kruskal–Wallace/Conover–Iman Tests | Mean MoA [hZT] | SDev MoA [hZT] | Qp [%] | General Stability [Arbitrary Units] |
---|---|---|---|---|
C3H | ||||
(mouse ID) | ||||
(19) ● | 16.10 | 0.4691 | 37.0 | 78.8 |
(30) | 15.41 | 0.1969 | 45.4 | 230.6 |
(4) | 17.25 | 0.4369 | 34.2 | 78.3 |
(3) | 16.25 | 0.3379 | 39.4 | 116.6 |
(2) | 16.75 | 0.4983 | 37.9 | 76.9 |
(1) | 16.42 | 0.3935 | 34.4 | 87.4 |
C3H MT 1 KO | ||||
(mouse ID) | ||||
(17) | 15.83 | 0.3878 | 41.1 | 106.0 |
(16) | 16.17 | 0.4522 | 37.2 | 82.3 |
(15) | 16.58 | 0.4931 | 42.5 | 86.2 |
(13) | 16.75 | 0.4397 | 36.3 | 82.6 |
(24) | 16.75 | 0.3809 | 37.5 | 98.4 |
(14) | 16.92 | 0.2500 | 40.0 | 160.0 |
C3H MT 2 KO | ||||
(mouse ID) | ||||
(7) | 15.42 | 0.3472 | 40.3 | 116.1 |
(6 | 15.83 | 0.3206 | 45.1 | 140.7 |
(18) | 17.25 | 0.4346 | 30.4 | 69.9 |
(5) | 16.25 | 0.6314 | 37.4 | 59.3 |
(8) | 16.50 | 0.5503 | 29.7 | 53.9 |
(23) | 16.12 | 0.1476 | 44.0 | 298.1 |
C3H MT 1,2 KO | ||||
(mouse ID) | ||||
(12) | 16.42 | 0.3685 | 39.2 | 106.4 |
(20) ● | 17.67 | 0.8963 | 27.3 | 30.5 |
(9 | 16.67 | 0.6432 | 23.4 | 36.4 |
(10) | 17.08 | 0.8579 | 24.3 | 28.3 |
(11) | 16.42 | 0.7010 | 32.9 | 46.9 |
(21) | 16.42 | 0.3839 | 28.9 | 75.3 |
C57Bl | ||||
(mouse ID) | ||||
(16) | 17.41 | 1.1429 | 31.5 | 27.6 |
(13) ● | 22.58 | 1.1279 | 27.0 | 23.9 |
(11) | 17.50 | 0.5294 | 28.1 | 53.1 |
(7) | 20.75 | 1.8064 | 18.2 | 10.1 |
(12) | 18.67 | 1.6379 | 20.5 | 12.5 |
(10) | 20.25 | 1.0628 | 24.3 | 22.9 |
(26) | 17.00 | 0.4625 | 35.8 | 77.4 |
(25) | 17.50 | 0.5966 | 35.0 | 58.7 |
(27) | 17.33 | 1.1021 | 35.4 | 32.1 |
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Pfeffer, M.; Korf, H.-W.; Wicht, H. The Role of the Melatoninergic System in Light-Entrained Behavior of Mice. Int. J. Mol. Sci. 2017, 18, 530. https://doi.org/10.3390/ijms18030530
Pfeffer M, Korf H-W, Wicht H. The Role of the Melatoninergic System in Light-Entrained Behavior of Mice. International Journal of Molecular Sciences. 2017; 18(3):530. https://doi.org/10.3390/ijms18030530
Chicago/Turabian StylePfeffer, Martina, Horst-Werner Korf, and Helmut Wicht. 2017. "The Role of the Melatoninergic System in Light-Entrained Behavior of Mice" International Journal of Molecular Sciences 18, no. 3: 530. https://doi.org/10.3390/ijms18030530