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Int. J. Mol. Sci. 2017, 18(2), 234;

RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China
Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
Author to whom correspondence should be addressed.
Academic Editor: Constantinos Stathopoulos
Received: 30 November 2016 / Revised: 11 January 2017 / Accepted: 13 January 2017 / Published: 10 February 2017
(This article belongs to the Section Biochemistry)
PDF [1078 KB, uploaded 10 February 2017]


The silkworm Bombyx mori is an economically important species. White muscardine caused by Beauveria bassiana is the main fungal disease in sericulture, and understanding the silkworm responses to B. bassiana infection is of particular interest. Herein, we investigated the molecular mechanisms underlying these responses in two silkworm strains Haoyue (HY, sensitive to B. bassiana) and Kang 8 (K8, resistant to B. bassiana) using an RNA-seq approach. For each strain, three biological replicates for immersion treatment, two replicates for injection treatment and three untreated controls were collected to generate 16 libraries for sequencing. Differentially expressed genes (DEGs) between treated samples and untreated controls, and between the two silkworm strains, were identified. DEGs and the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the two strains exhibited an obvious difference. Several genes encoding cuticle proteins, serine proteinase inhibitors (SPI) and antimicrobial peptides (AMP) and the drug metabolism pathway involved in toxin detoxification were considered to be related to the resistance of K8 to B. bassiana. These results revealed insight into the resistance and susceptibility of two silkworm strains against B. bassiana infection and provided a roadmap for silkworm molecular breeding to enhance its resistance to B. bassiana. View Full-Text
Keywords: silkworm; RNA-seq; Beauveria bassiana; resistance silkworm; RNA-seq; Beauveria bassiana; resistance

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Xing, D.; Yang, Q.; Jiang, L.; Li, Q.; Xiao, Y.; Ye, M.; Xia, Q. RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection. Int. J. Mol. Sci. 2017, 18, 234.

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