Insights into Insulin Fibril Assembly at Physiological and Acidic pH and Related Amyloid Intrinsic Fluorescence
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Materials
4.2. Insulin Preparation and Amyloid Fibril Formation
4.3. Transmission Electronic Microscopy (TEM)
4.4. Fluorescence Measurements
4.5. Two Photons Microscopy Measurements
4.6. Circular Dichroism Measurements
4.7. Fourier-Transform Infrared Measurements
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Protein | Secondary Structure (%) | |||
---|---|---|---|---|
Intermolecular β-sheet 1611–1630 cm−1 | Intramolecular β-sheet 1615–1638 cm−1 | α-helix 1640–1655 cm−1 | Disordered/β-turn 1658–1686 cm−1 | |
Native insulin | 0 | 0 | 60 | 40 |
Fibrils pH 7.0 | 18 | 56 | 5 | 21 |
Fibrils pH 1.8 | 76 | 0 | 7 | 17 |
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Iannuzzi, C.; Borriello, M.; Portaccio, M.; Irace, G.; Sirangelo, I. Insights into Insulin Fibril Assembly at Physiological and Acidic pH and Related Amyloid Intrinsic Fluorescence. Int. J. Mol. Sci. 2017, 18, 2551. https://doi.org/10.3390/ijms18122551
Iannuzzi C, Borriello M, Portaccio M, Irace G, Sirangelo I. Insights into Insulin Fibril Assembly at Physiological and Acidic pH and Related Amyloid Intrinsic Fluorescence. International Journal of Molecular Sciences. 2017; 18(12):2551. https://doi.org/10.3390/ijms18122551
Chicago/Turabian StyleIannuzzi, Clara, Margherita Borriello, Marianna Portaccio, Gaetano Irace, and Ivana Sirangelo. 2017. "Insights into Insulin Fibril Assembly at Physiological and Acidic pH and Related Amyloid Intrinsic Fluorescence" International Journal of Molecular Sciences 18, no. 12: 2551. https://doi.org/10.3390/ijms18122551