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Involvement of Opioid System, TRPM8, and ASIC Receptors in Antinociceptive Effect of Arrabidaea brachypoda (DC) Bureau

São Paulo State University (UNESP), Biosciences Institute, Department of Physiology, Botucatu 18618-970, SP, Brazil
Federal University of Maranhão, Department of Chemistry, Av. dos Portugueses, 1966—Bacanga, São Luís 65080-805, MA, Brazil
Clinical Pharmacology and Gastroenterology Unit (USF), Bragança Paulista 12916-900, SP, Brazil
School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, CMU–Rue Michel-Servet 1, CH-1211 Geneva 4, Switzerland
Federal University of Santa Catarina, Laboratory of Neurobiology of Pain and Inflammation, Department of Physiological Sciences, Center of Biological Sciences, Trindade, Florianopolis 88040-900, SC, Brazil
São Paulo State University (UNESP), Biosciences Institute, São Vicente 11330-900, SP, Brazil
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(11), 2304;
Received: 3 October 2017 / Revised: 27 October 2017 / Accepted: 1 November 2017 / Published: 2 November 2017
(This article belongs to the Special Issue Bioactive Phenolics and Polyphenols 2018)
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Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as “cipó-una”, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10–100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief. View Full-Text
Keywords: Arrabidaea brachypoda (DC) Bureau; Bignoniaceae; antinociceptive effect; pain Arrabidaea brachypoda (DC) Bureau; Bignoniaceae; antinociceptive effect; pain

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rodrigues, V.P.; Rocha, C.Q.; Périco, L.L.; Santos, R.D.C.; Ohara, R.; Nishijima, C.M.; Ferreira Queiroz, E.; Wolfender, J.-L.; Rocha, L.R.M.; Santos, A.R.S.; Vilegas, W.; Hiruma-Lima, C.A. Involvement of Opioid System, TRPM8, and ASIC Receptors in Antinociceptive Effect of Arrabidaea brachypoda (DC) Bureau. Int. J. Mol. Sci. 2017, 18, 2304.

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