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Int. J. Mol. Sci. 2017, 18(10), 2177; https://doi.org/10.3390/ijms18102177

Oil Body-Bound Oleosin-rhFGF-10: A Novel Drug Delivery System that Improves Skin Penetration to Accelerate Wound Healing and Hair Growth in Mice

1
College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China
2
Wenzhou Biomedical Innovation Center, Wenzhou University, Wenzhou 325035, China
3
College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 25 September 2017 / Revised: 13 October 2017 / Accepted: 15 October 2017 / Published: 18 October 2017
(This article belongs to the Special Issue Guided Amphiphile and Lipid Membrane Self-Assembly)
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Abstract

Recombinant human fibroblast growth factor 10 (rhFGF-10) is frequently used to treat patients with skin injuries. It can also promote hair growth. However, the effective application of rhFGF-10 is limited because of its poor stability and transdermal absorption. In this study, polymerase chain reaction (PCR) and Southern blotting were used to identify transgenic safflowers carrying a gene encoding an oleosin-rhFGF-10 fusion protein. The size and structural integrity of oleosin-rhFGF-10 in oil bodies extracted from transgenic safflower seeds was characterized by polyacrylamide gel electrophoresis and western blotting. Oil body extracts containing oleosin-rhFGF-10 were topically applied to mouse skin. The absorption of oleosin-rhFGF-10 was studied by immunohistochemistry. Its efficiency in promoting wound healing and hair regeneration were evaluated in full thickness wounds and hair growth assays. We identified a safflower line that carried the transgene and expressed a 45 kDa oleosin-rhFGF-10 protein. Oil body-bound oleosin-rhFGF-10 was absorbed by the skin with higher efficiency and speed compared with prokaryotically-expressed rhFGF-10. Oleosin-rhFGF-10 also enhanced wound closure and promoted hair growth better than rhFGF-10. The application of oleosin-rhFGF-10 in oil bodies promoted its delivery through the skin, providing a basis for improved therapeutic effects in enhancing wound healing and hair growth. View Full-Text
Keywords: oil body; oleosin-rhFGF-10; skin penetration; wound healing; hair growth oil body; oleosin-rhFGF-10; skin penetration; wound healing; hair growth
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Li, W.; Yang, J.; Cai, J.; Wang, H.; Tian, H.; Huang, J.; Qiang, W.; Zhang, L.; Li, H.; Li, X.; Jiang, C. Oil Body-Bound Oleosin-rhFGF-10: A Novel Drug Delivery System that Improves Skin Penetration to Accelerate Wound Healing and Hair Growth in Mice. Int. J. Mol. Sci. 2017, 18, 2177.

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