Sulforaphane, a Dietary Isothiocyanate, Induces G2/M Arrest in Cervical Cancer Cells through CyclinB1 Downregulation and GADD45β/CDC2 Association
Abstract
:1. Introduction
2. Results and Discussion
2.1. SFN Inhibits Cell Survival in Cervical Cancer Cell Lines
2.2. Non-SFN-Induced Apoptosis/Necrosis of Cx, CxWj and HeLa Cell Lines
2.3. SFN Induced the Accumulation of Cells in the G2/M Phase
2.4. Effects of SFN on the Mitotic Index
2.5. G2/M Phase Arrest in SFN-Treated Cells via Cyclin B1 Down-Regulation and GADD45β Up-Regulation
2.6. Effects of SFN on the CDC2 and CDC25C in HeLa Cells
3. Experimental Section
3.1. Materials
3.2. Cells
3.3. Cell Proliferation Assay
3.4. Measurement of Apoptosis
3.5. Caspase 3 Activity Assay
3.6. Mitochondrial Membrane Potential (MMP)
3.7. Cell Cycle Analysis
3.8. Mitotic Index Analysis
3.9. Western Blot Assay
3.10. Co-Immunoprecipitation (Co-IP)
3.11. Real-Time PCR
3.12. Quantitative Real-Time PCR
3.13. Statistical Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Cheng, Y.-M.; Tsai, C.-C.; Hsu, Y.-C. Sulforaphane, a Dietary Isothiocyanate, Induces G2/M Arrest in Cervical Cancer Cells through CyclinB1 Downregulation and GADD45β/CDC2 Association. Int. J. Mol. Sci. 2016, 17, 1530. https://doi.org/10.3390/ijms17091530
Cheng Y-M, Tsai C-C, Hsu Y-C. Sulforaphane, a Dietary Isothiocyanate, Induces G2/M Arrest in Cervical Cancer Cells through CyclinB1 Downregulation and GADD45β/CDC2 Association. International Journal of Molecular Sciences. 2016; 17(9):1530. https://doi.org/10.3390/ijms17091530
Chicago/Turabian StyleCheng, Ya-Min, Ching-Chou Tsai, and Yi-Chiang Hsu. 2016. "Sulforaphane, a Dietary Isothiocyanate, Induces G2/M Arrest in Cervical Cancer Cells through CyclinB1 Downregulation and GADD45β/CDC2 Association" International Journal of Molecular Sciences 17, no. 9: 1530. https://doi.org/10.3390/ijms17091530