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Int. J. Mol. Sci. 2016, 17(8), 1243;

Femtosecond Laser Patterning of the Biopolymer Chitosan for Biofilm Formation

São Carlos Institute of Physics, University of São Paulo (USP), São Carlos 13566-590, SP, Brazil
Department of Materials Engineering, School of Engineering of São Carlos (USP), São Carlos 13563-120, SP, Brazil
Physics Department, Federal University of São Carlos (UFSCAR), São Carlos 13565-905, SP, Brazil
Faculdade de Ciencias Farmaceuticas, UNESP—Univ. Estadual Paulista, Campus Araraquara, Departamento de Analises Clinicas, Araraquara 14800-903, SP, Brazil
Authors to whom correspondence should be addressed.
Academic Editor: Hitoshi Sashiwa
Received: 16 June 2016 / Revised: 13 July 2016 / Accepted: 26 July 2016 / Published: 19 August 2016
(This article belongs to the Special Issue Chitins 2016)
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Controlling microbial growth is crucial for many biomedical, pharmaceutical and food industry applications. In this paper, we used a femtosecond laser to microstructure the surface of chitosan, a biocompatible polymer that has been explored for applications ranging from antimicrobial action to drug delivery. The influence of energy density on the features produced on chitosan was investigated by optical and atomic force microscopies. An increase in the hydrophilic character of the chitosan surface was attained upon laser micromachining. Patterned chitosan films were used to observe Staphylococcus aureus (ATCC 25923) biofilm formation, revealing an increase in the biofilm formation in the structured regions. Our results indicate that fs-laser micromachining is an attractive option to pattern biocompatible surfaces, and to investigate basic aspects of the relationship between surface topography and bacterial adhesion. View Full-Text
Keywords: fs-laser micromachining; micropatterning; chitosan; bacterial growth fs-laser micromachining; micropatterning; chitosan; bacterial growth

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Estevam-Alves, R.; Ferreira, P.H.D.; Coatrini, A.C.; Oliveira, O.N.; Fontana, C.R.; Mendonca, C.R. Femtosecond Laser Patterning of the Biopolymer Chitosan for Biofilm Formation. Int. J. Mol. Sci. 2016, 17, 1243.

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