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Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(7), 1016;

Screening of α-Tocopherol Transfer Protein Sensitive Genes in Human Hepatoma Cells (HepG2)

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
The authors contributed equally to the work.
Author to whom correspondence should be addressed.
Academic Editor: Johannes Haybaeck
Received: 15 May 2016 / Revised: 19 June 2016 / Accepted: 21 June 2016 / Published: 27 June 2016
(This article belongs to the Section Physical Chemistry, Theoretical and Computational Chemistry)
Full-Text   |   PDF [1349 KB, uploaded 27 June 2016]   |  


α-Tocopherol transfer protein (α-TTP) is a ~32 kDa protein expressed mainly in hepatocytes. The major function of the protein is to bind specifically to α-tocopherol and, together, the complex transfers from late lysosomes to the cell membrane. A previous study indicated that some factors might be required in the transferring process. However, there is little information available about the potential transferring factors. In addition, there remains much to learn about other physiological processes which α-TTP might participate in. Thus, in this study a human α-TTP eukaryotic expression vector was successfully constructed and expressed in human hepatoma cells (HepG2). The sensitive genes related to α-TTP were then screened by microarray technology. Results showed that expression of the vector in HepG2 cells led to the identification of 323 genes showing differential expression. The differentially expressed transcripts were divided into four main categories, including (1) cell inflammation; (2) cell cycle and cell apoptosis; (3) cell signaling and gene regulation; and (4) cellular movement. A few cellular movement related transcripts were selected and verified by quantitative real-time PCR. Expressions of some were significantly increased in α-TTP-expressed group, which indicated that these factors were likely to play a role in the transferring process. View Full-Text
Keywords: α-tocopherol transfer protein; vitamin E; sensitive genes; microarray α-tocopherol transfer protein; vitamin E; sensitive genes; microarray

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Qu, Y.-H.; Fu, J.-C.; Liu, K.; Zuo, Z.-Y.; Jia, H.-N.; Ma, Y.; Luo, H.-L. Screening of α-Tocopherol Transfer Protein Sensitive Genes in Human Hepatoma Cells (HepG2). Int. J. Mol. Sci. 2016, 17, 1016.

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