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Open AccessArticle

The AaDREB1 Transcription Factor from the Cold-Tolerant Plant Adonis amurensis Enhances Abiotic Stress Tolerance in Transgenic Plant

College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, Jilin, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Jianhua Zhu
Int. J. Mol. Sci. 2016, 17(4), 611; https://doi.org/10.3390/ijms17040611
Received: 26 November 2015 / Revised: 15 April 2016 / Accepted: 18 April 2016 / Published: 22 April 2016
(This article belongs to the Special Issue Gene–Environment Interactions)
Dehydration-responsive element binding (DREB) transcription factors (TFs) play important roles in the regulation of plant resistance to environmental stresses and can specifically bind to dehydration-responsive element/C-repeat element (DRE/CRT) proteins (G/ACCGAC) and activate expression of many stress-inducible genes. Here, we cloned and characterized a novel gene (AaDREB1) encoding the DREB1 transcription factor from the cold-tolerant plant Adonis amurensis. Quantitative real-time (qRT)-PCR results indicated that AaDREB1 expression was induced by salt, drought, cold stress, and abscisic acid application. A yeast one-hybrid assay demonstrated that AaDREB1 encodes a transcription activator and specifically binds to DRE/CRT. Furthermore, transgenic Arabidopsis and rice harboring AaDREB1 showed enhanced tolerance to salt, drought, and low temperature. These results indicated that AaDREB1 might be useful in genetic engineering to improve plant stress tolerance. View Full-Text
Keywords: AaDREB1; abiotic stress tolerance; Adonis amurensis; transgenic plant AaDREB1; abiotic stress tolerance; Adonis amurensis; transgenic plant
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Zong, J.-M.; Li, X.-W.; Zhou, Y.-H.; Wang, F.-W.; Wang, N.; Dong, Y.-Y.; Yuan, Y.-X.; Chen, H.; Liu, X.-M.; Yao, N.; Li, H.-Y. The AaDREB1 Transcription Factor from the Cold-Tolerant Plant Adonis amurensis Enhances Abiotic Stress Tolerance in Transgenic Plant. Int. J. Mol. Sci. 2016, 17, 611.

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