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Open AccessArticle

Prediction of Toxin Genes from Chinese Yellow Catfish Based on Transcriptomic and Proteomic Sequencing

by 1,2,†, 1,†, 2,†, 3, 3, 2, 2, 3, 3, 2,*, 1,2,* and 2,3,4,*
Section on Marine biobank, China National Genebank, Shenzhen 518083, China
BGI-Shenzhen, Shenzhen 518083, China
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, Shenzhen 518083, China
Center for Marine Research, College of Life Sciences, Shenzhen University, Shenzhen 518060, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Jun Li and Li Lin
Int. J. Mol. Sci. 2016, 17(4), 556;
Received: 14 March 2016 / Revised: 6 April 2016 / Accepted: 7 April 2016 / Published: 13 April 2016
(This article belongs to the Special Issue Fish Molecular Biology)
Fish venom remains a virtually untapped resource. There are so few fish toxin sequences for reference, which increases the difficulty to study toxins from venomous fish and to develop efficient and fast methods to dig out toxin genes or proteins. Here, we utilized Chinese yellow catfish (Pelteobagrus fulvidraco) as our research object, since it is a representative species in Siluriformes with its venom glands embedded in the pectoral and dorsal fins. In this study, we set up an in-house toxin database and a novel toxin-discovering protocol to dig out precise toxin genes by combination of transcriptomic and proteomic sequencing. Finally, we obtained 15 putative toxin proteins distributed in five groups, namely Veficolin, Ink toxin, Adamalysin, Za2G and CRISP toxin. It seems that we have developed a novel bioinformatics method, through which we could identify toxin proteins with high confidence. Meanwhile, these toxins can also be useful for comparative studies in other fish and development of potential drugs. View Full-Text
Keywords: Chinese yellow catfish; venom; transcriptome; proteome Chinese yellow catfish; venom; transcriptome; proteome
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MDPI and ACS Style

Xie, B.; Li, X.; Lin, Z.; Ruan, Z.; Wang, M.; Liu, J.; Tong, T.; Li, J.; Huang, Y.; Wen, B.; Sun, Y.; Shi, Q. Prediction of Toxin Genes from Chinese Yellow Catfish Based on Transcriptomic and Proteomic Sequencing. Int. J. Mol. Sci. 2016, 17, 556.

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