Autoimmune Regulator Expression in DC2.4 Cells Regulates the NF-κB Signaling and Cytokine Expression of the Toll-Like Receptor 3 Pathway
Abstract
:1. Introduction
2. Results
2.1. Establishment of Stable Aire-Expressing Transfectants (Stable Aire Expression in DC2.4 Cells)
2.2. Aire Regulates TLR Expression in Stable Aire-Expressing DC2.4 Cells
2.3. Aire Influences the Phenotype of DC2.4 Cells
2.4. Aire Regulates Cytokine Expression Downstream of the TLR3 Pathway
2.5. Aire Modifies Key TLR3 Pathway Molecules in DC2.4
3. Discussion
4. Experimental Section
4.1. Cells and Animals
4.2. Cell Culture and Ligand Stimulation
4.3. Transfection
4.4. Quantitative Real-Time PCR (RT-qPCR)
4.5. Flow Cytometry (FCM)
4.6. Western Blotting
4.7. Immunofluorescence Staining
4.8. ELISA
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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MFI % | GFP/DC | GFP-Aire/DC | ||
---|---|---|---|---|
Control | Poly (I:C) | Control | Poly (I:C) | |
CD40 | 0.50 ± 0.25 | 2.60 ± 0.18 1 | 0.64 ± 0.22 | 0.98 ± 0.27 2,3 |
1.67 ± 0.64 | 8.35 ± 0.35 2 | 6.61 ± 0.13 | 7.34 ± 1.02 | |
CD80 | 51.60 ± 12.16 | 69.60 ± 0.64 | 26.20 ± 8.76 2 | 14.51 ± 10.57 3 |
30.43 ± 7.36 | 43.67 ± 1.37 | 25.99 ± 0.02 | 8.08 ± 4.57 4 | |
CD83 | 17.07 ± 0.49 | 8.46 ± 0.85 1 | 13.60 ± 1.70 | 4.08 ± 3.29 3 |
13.75 ± 3.17 | 13.18 ± 9.03 | 11.75 ± 1.27 | 9.02 ± 3.89 | |
CD11C | 7.93 ± 0.49 | 11.70 ± 0.28 2 | 6.69 ± 0.22 | 5.03 ± 0.18 4 |
15.59 ± 5.51 | 19.60 ± 0.87 | 17.41 ± 2.75 | 16.14 ± 0.20 | |
CD86 | 1.82 ± 0.39 | 2.70 ± 1.00 2 | 2.89 ± 0.25 2 | 2.34 ± 0.19 2 |
4.03 ± 0.38 | 5.88 ± 1.75 2 | 3.59 ± 0.05 2 | 6.60 ± 0.68 | |
MHC II | 0.29 ± 0.14 | 1.09 ± 0.11 2 | 0.42 ± 0.29 | 0.42 ± 0.16 3 |
0.62 ± 0.41 | 1.37 ± 0.43 | 2.42 ± 0.01 1 | 2.22 ± 1.93 |
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Sun, J.; Niu, K.; Fu, H.; Li, H.; Li, Y.; Yang, W. Autoimmune Regulator Expression in DC2.4 Cells Regulates the NF-κB Signaling and Cytokine Expression of the Toll-Like Receptor 3 Pathway. Int. J. Mol. Sci. 2016, 17, 2002. https://doi.org/10.3390/ijms17122002
Sun J, Niu K, Fu H, Li H, Li Y, Yang W. Autoimmune Regulator Expression in DC2.4 Cells Regulates the NF-κB Signaling and Cytokine Expression of the Toll-Like Receptor 3 Pathway. International Journal of Molecular Sciences. 2016; 17(12):2002. https://doi.org/10.3390/ijms17122002
Chicago/Turabian StyleSun, Jitong, Kunwei Niu, Haiying Fu, Haijun Li, Yi Li, and Wei Yang. 2016. "Autoimmune Regulator Expression in DC2.4 Cells Regulates the NF-κB Signaling and Cytokine Expression of the Toll-Like Receptor 3 Pathway" International Journal of Molecular Sciences 17, no. 12: 2002. https://doi.org/10.3390/ijms17122002