Trans-Stilbenes in Commercial Grape Juices: Quantification Using HPLC Approaches
Faculty of Pharmacy, Department of Analytical Chemistry, Nutrition and Food Science, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, Spain
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Academic Editors: Maurizio Battino and Francesca Giampieri
Int. J. Mol. Sci. 2016, 17(10), 1769; https://doi.org/10.3390/ijms17101769
Received: 22 September 2016 / Revised: 17 October 2016 / Accepted: 18 October 2016 / Published: 24 October 2016
(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2016)
Trans-stilbenes belong to the group of polyphenolic phytoalexins, and occur in many plant foods. These compounds have received great attention by researchers due to their well-known beneficial health effects. In the present study a chromatographic method that comprises the use of variable wavelength (VWD) and fluorescence (FLD) detectors in series for the analysis of trans-stilbenes is presented. The relation of peak-area obtained with both detectors is proposed as an alternative and complementary approach for the rapid identification of these phenolic compounds. The proposed method was applied to determine trans-stilbenes in commercial fruit juices. Trans-piceid was the most common trans-stilbene found in the samples analyzed. The method was validated in terms of linearity, sensitivity and repeatability. Appropriate sensitivity and good linearity (r2 > 0.9991) were achieved.
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Keywords:
trans-stilbenes; commercial fruit juices; high-performance liquid chromatography; variable wavelength detector; fluorescence detector
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MDPI and ACS Style
López-Hernández, J.; Rodríguez-Bernaldo de Quirós, A. Trans-Stilbenes in Commercial Grape Juices: Quantification Using HPLC Approaches. Int. J. Mol. Sci. 2016, 17, 1769.
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