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Open AccessArticle

Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)

by 1,2,3, 1,2,3, 4, 1, 1,2,3, 5, 5 and 1,2,3,*
1
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
2
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China
3
Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China
4
Department of Public Health Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA
5
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Academic Editor: Helmut Segner
Int. J. Mol. Sci. 2016, 17(10), 1693; https://doi.org/10.3390/ijms17101693
Received: 4 July 2016 / Revised: 26 September 2016 / Accepted: 27 September 2016 / Published: 9 October 2016
(This article belongs to the Section Biochemistry)
Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp (Cyprinus carpio) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region of trim32 was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant decrease of SVCV production as compared to the control group. These results suggested a potentially important role of common carp TRIM32 in enhancing host immune response during SVCV infection both in vivo and in vitro. View Full-Text
Keywords: TRIM32; spring viraemia of carp virus; common carp TRIM32; spring viraemia of carp virus; common carp
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MDPI and ACS Style

Wang, Y.; Li, Z.; Lu, Y.; Hu, G.; Lin, L.; Zeng, L.; Zhou, Y.; Liu, X. Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio). Int. J. Mol. Sci. 2016, 17, 1693. https://doi.org/10.3390/ijms17101693

AMA Style

Wang Y, Li Z, Lu Y, Hu G, Lin L, Zeng L, Zhou Y, Liu X. Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio). International Journal of Molecular Sciences. 2016; 17(10):1693. https://doi.org/10.3390/ijms17101693

Chicago/Turabian Style

Wang, Yeda; Li, Zeming; Lu, Yuanan; Hu, Guangfu; Lin, Li; Zeng, Lingbing; Zhou, Yong; Liu, Xueqin. 2016. "Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)" Int. J. Mol. Sci. 17, no. 10: 1693. https://doi.org/10.3390/ijms17101693

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