Next Article in Journal
Validated HPAEC-PAD Method for the Determination of Fully Deacetylated Chitooligosaccharides
Next Article in Special Issue
Nitrogen Removal from Landfill Leachate by Microalgae
Previous Article in Journal
Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)
Previous Article in Special Issue
Biodiesel Production from Chlorella protothecoides Oil by Microwave-Assisted Transesterification
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(10), 1692;

Microalgae Cultivation on Anaerobic Digestate of Municipal Wastewater, Sewage Sludge and Agro-Waste

Dipartimento di Biotecnologie, Università di Verona, Strada le Grazie 15, 37134 Verona, Italy
Author to whom correspondence should be addressed.
Academic Editor: Yongsheng Chen
Received: 30 July 2016 / Revised: 16 September 2016 / Accepted: 29 September 2016 / Published: 10 October 2016
(This article belongs to the Special Issue Algae Based Bio-Renewable Energy for Sustainability)
PDF [2945 KB, uploaded 10 October 2016]


Microalgae are fast-growing photosynthetic organisms which have the potential to be exploited as an alternative source of liquid fuels to meet growing global energy demand. The cultivation of microalgae, however, still needs to be improved in order to reduce the cost of the biomass produced. Among the major costs encountered for algal cultivation are the costs for nutrients such as CO2, nitrogen and phosphorous. In this work, therefore, different microalgal strains were cultivated using as nutrient sources three different anaerobic digestates deriving from municipal wastewater, sewage sludge or agro-waste treatment plants. In particular, anaerobic digestates deriving from agro-waste or sewage sludge treatment induced a more than 300% increase in lipid production per volume in Chlorella vulgaris cultures grown in a closed photobioreactor, and a strong increase in carotenoid accumulation in different microalgae species. Conversely, a digestate originating from a pilot scale anaerobic upflow sludge blanket (UASB) was used to increase biomass production when added to an artificial nutrient-supplemented medium. The results herein demonstrate the possibility of improving biomass accumulation or lipid production using different anaerobic digestates. View Full-Text
Keywords: microalgae; anaerobic digestate; biofuels; photosynthesis microalgae; anaerobic digestate; biofuels; photosynthesis

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Zuliani, L.; Frison, N.; Jelic, A.; Fatone, F.; Bolzonella, D.; Ballottari, M. Microalgae Cultivation on Anaerobic Digestate of Municipal Wastewater, Sewage Sludge and Agro-Waste. Int. J. Mol. Sci. 2016, 17, 1692.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top