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Int. J. Mol. Sci. 2016, 17(10), 1632;

Global Transcriptomic Analysis of Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana

College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei 10617, Taiwan
Graduate Institute of Biotechnology, Chinese Culture University, Taipei 11114, Taiwan
Authors to whom correspondence should be addressed.
Academic Editor: María Serrano
Received: 31 July 2016 / Revised: 22 August 2016 / Accepted: 13 September 2016 / Published: 26 September 2016
(This article belongs to the Section Molecular Plant Sciences)
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Among 18 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase homologous genes existing in the banana genome there are two genes, Mh-ACO1 and Mh-ACO2, that participate in banana fruit ripening. To better understand the physiological functions of Mh-ACO1 and Mh-ACO2, two hairpin-type siRNA expression vectors targeting both the Mh-ACO1 and Mh-ACO2 were constructed and incorporated into the banana genome by Agrobacterium-mediated transformation. The generation of Mh-ACO1 and Mh-ACO2 RNAi transgenic banana plants was confirmed by Southern blot analysis. To gain insights into the functional diversity and complexity between Mh-ACO1 and Mh-ACO2, transcriptome sequencing of banana fruits using the Illumina next-generation sequencer was performed. A total of 32,093,976 reads, assembled into 88,031 unigenes for 123,617 transcripts were obtained. Significantly enriched Gene Oncology (GO) terms and the number of differentially expressed genes (DEGs) with GO annotation were ‘catalytic activity’ (1327, 56.4%), ‘heme binding’ (65, 2.76%), ‘tetrapyrrole binding’ (66, 2.81%), and ‘oxidoreductase activity’ (287, 12.21%). Real-time RT-PCR was further performed with mRNAs from both peel and pulp of banana fruits in Mh-ACO1 and Mh-ACO2 RNAi transgenic plants. The results showed that expression levels of genes related to ethylene signaling in ripening banana fruits were strongly influenced by the expression of genes associated with ethylene biosynthesis. View Full-Text
Keywords: fruit ripening; ethylene production; genetic transformation; RNA interference fruit ripening; ethylene production; genetic transformation; RNA interference

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Xia, Y.; Kuan, C.; Chiu, C.-H.; Chen, X.-J.; Do, Y.-Y.; Huang, P.-L. Global Transcriptomic Analysis of Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana. Int. J. Mol. Sci. 2016, 17, 1632.

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