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Open AccessArticle

Proteomic Analysis of Hylocereus polyrhizus Reveals Metabolic Pathway Changes

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
General Station of the Administration of Seeds Guangdong Province, Guangzhou 510500, China
Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA
Dongguan Institute of Forest Science, Dongguan 523106, China
Authors to whom correspondence should be addressed.
Academic Editor: Setsuko Komatsu
Int. J. Mol. Sci. 2016, 17(10), 1606;
Received: 3 July 2016 / Revised: 3 September 2016 / Accepted: 13 September 2016 / Published: 28 September 2016
(This article belongs to the Special Issue Plant Proteomic Research)
Red dragon fruit or red pitaya (Hylocereus polyrhizus) is the only edible fruit that contains betalains. The color of betalains ranges from red and violet to yellow in plants. Betalains may also serve as an important component of health-promoting and disease-preventing functional food. Currently, the biosynthetic and regulatory pathways for betalain production remain to be fully deciphered. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analyses were used to reveal the molecular mechanism of betalain biosynthesis in H. polyrhizus fruits at white and red pulp stages, respectively. A total of 1946 proteins were identified as the differentially expressed between the two samples, and 936 of them were significantly highly expressed at the red pulp stage of H. polyrhizus. RNA-seq and iTRAQ analyses showed that some transcripts and proteins were positively correlated; they belonged to “phenylpropanoid biosynthesis”, “tyrosine metabolism”, “flavonoid biosynthesis”, “ascorbate and aldarate metabolism”, “betalains biosynthesis” and “anthocyanin biosynthesis”. In betalains biosynthesis pathway, several proteins/enzymes such as polyphenol oxidase, CYP76AD3 and 4,5-dihydroxy-phenylalanine (DOPA) dioxygenase extradiol-like protein were identified. The present study provides a new insight into the molecular mechanism of the betalain biosynthesis at the posttranscriptional level. View Full-Text
Keywords: Hylocereus polyrhizus; isobaric tags for relative and absolute quantitation (iTRAQ); transcriptome; betalain biosynthesis Hylocereus polyrhizus; isobaric tags for relative and absolute quantitation (iTRAQ); transcriptome; betalain biosynthesis
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Hua, Q.; Zhou, Q.; Gan, S.; Wu, J.; Chen, C.; Li, J.; Ye, Y.; Zhao, J.; Hu, G.; Qin, Y. Proteomic Analysis of Hylocereus polyrhizus Reveals Metabolic Pathway Changes. Int. J. Mol. Sci. 2016, 17, 1606.

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