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Int. J. Mol. Sci. 2016, 17(10), 1559;

Preparation, Characterization and in Vivo Antimycobacterial Studies of Panchovillin-Chitosan Nanocomposites

Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, 14115 Dar es Salaam, Tanzania
National Institute for Medical Research (NIMR), P.O. Box 9653, 14115 Dar es Salaam, Tanzania
Author to whom correspondence should be addressed.
Academic Editor: Már Másson
Received: 18 July 2016 / Revised: 23 August 2016 / Accepted: 30 August 2016 / Published: 27 September 2016
(This article belongs to the Special Issue Drug Delivery and Antimicrobial Agents)
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Chitosan (CS, molecular weight 20.2 kDa, degree of deacylation (DD) 73.31%) was successfully obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the bioactive natural product, panchovillin (PANV), isolated from Erythrina schliebenii, on a chitosan-tripolyphosphate (CS/TPP) nano-framework was achieved by ionotropic gelation. Characterization of pure CS, CS/TPP and PANV-CS/TPP nanocomposites was performed by FTIR, SEM and XRD. The molecular weight of chitosan and the thermal stability of the materials were determined by MALDI-TOF-MS and simultaneous thermal analyzer (STA)/DTG, respectively. The respective encapsulation efficiency and loading capacity of the PANV were found to be 70% and 0.36%. The in vitro release studies showed an initial burst of 42% of PANV in the first six hours. This was followed by a slow and sustained release up to 72 h. The in vivo antimycobacterial activities of both PANV and PANV-CS/TPP nanocomposite against Mycobacterium indicus pranii (MIP) using Galleria mellonella larvae as an in vivo infection model are reported in this paper. View Full-Text
Keywords: panchovillin; chitosan; nanocomposites; antimycobacterial; Galleria mellonella panchovillin; chitosan; nanocomposites; antimycobacterial; Galleria mellonella

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Rwegasila, E.; Mubofu, E.B.; Nyandoro, S.S.; Erasto, P.; Munissi, J.J.E. Preparation, Characterization and in Vivo Antimycobacterial Studies of Panchovillin-Chitosan Nanocomposites. Int. J. Mol. Sci. 2016, 17, 1559.

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