Next Article in Journal
Multiple Factors Drive Replicating Strand Composition Bias in Bacterial Genomes
Next Article in Special Issue
Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris
Previous Article in Journal
Critical Issues in the Study of Magnesium Transport Systems and Magnesium Deficiency Symptoms in Plants
Previous Article in Special Issue
Effects of Non-Natural Amino Acid Incorporation into the Enzyme Core Region on Enzyme Structure and Function
Open AccessReview

Rational Protein Engineering Guided by Deep Mutational Scanning

Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Qiang “Shawn” Chen
Int. J. Mol. Sci. 2015, 16(9), 23094-23110; https://doi.org/10.3390/ijms160923094
Received: 14 August 2015 / Revised: 4 September 2015 / Accepted: 13 September 2015 / Published: 23 September 2015
(This article belongs to the Special Issue Protein Engineering)
Sequence–function relationship in a protein is commonly determined by the three-dimensional protein structure followed by various biochemical experiments. However, with the explosive increase in the number of genome sequences, facilitated by recent advances in sequencing technology, the gap between protein sequences available and three-dimensional structures is rapidly widening. A recently developed method termed deep mutational scanning explores the functional phenotype of thousands of mutants via massive sequencing. Coupled with a highly efficient screening system, this approach assesses the phenotypic changes made by the substitution of each amino acid sequence that constitutes a protein. Such an informational resource provides the functional role of each amino acid sequence, thereby providing sufficient rationale for selecting target residues for protein engineering. Here, we discuss the current applications of deep mutational scanning and consider experimental design. View Full-Text
Keywords: deep mutational scanning; next generation sequencing; high-throughput screening; protein engineering deep mutational scanning; next generation sequencing; high-throughput screening; protein engineering
Show Figures

Figure 1

MDPI and ACS Style

Shin, H.; Cho, B.-K. Rational Protein Engineering Guided by Deep Mutational Scanning. Int. J. Mol. Sci. 2015, 16, 23094-23110.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop