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Article

Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration

1
School of Clinical Dentistry, University of Sheffield, Sheffield S10 2TA, UK
2
Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin 10129, Italy
3
School of Pharmacy, Royal College of Surgeons in Ireland, Dublin 2, Ireland
4
Division of Biology, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin 2, Ireland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Bing Yan
Int. J. Mol. Sci. 2015, 16(9), 20492-20510; https://doi.org/10.3390/ijms160920492
Received: 21 July 2015 / Revised: 5 August 2015 / Accepted: 7 August 2015 / Published: 28 August 2015
(This article belongs to the Special Issue Bioactive Nanoparticles)
Biodegradable poly(lactide-co-glycolide) (PLGA) nanoparticles, containing human parathyroid hormone (PTH (1–34)), prepared by a modified double emulsion-solvent diffusion-evaporation method, were incorporated in porous freeze-dried chitosan-gelatin (CH-G) scaffolds. The PTH-loaded nanoparticles (NPTH) were characterised in terms of morphology, size, protein loading, release kinetics and in vitro assessment of biological activity of released PTH and cytocompatibility studies against clonal human osteoblast (hFOB) cells. Structural integrity of incorporated and released PTH from nanoparticles was found to be intact by using Tris-tricine SDS-PAGE. In vitro PTH release kinetics from PLGA nanoparticles were characterised by a burst release followed by a slow release phase for 3–4 weeks. The released PTH was biologically active as evidenced by the stimulated release of cyclic AMP from hFOB cells as well as increased mineralisation studies. in vitro and cell studies demonstrated that the PTH bioactivity was maintained during the fabrication of PLGA nanoparticles and upon release. Finally, a content of 33.3% w/w NPTHs was incorporated in CH-G scaffolds, showing an intermittent release during the first 10 days and, followed by a controlled release over 28 days of observation time. The increased expression of Alkaline Phosphatase levels on hFOB cells further confirmed the activity of intermittently released PTH from scaffolds. View Full-Text
Keywords: bone tissue; nanoparticles; parathyroid hormone; poly(lactide-co-glycolide); scaffolds bone tissue; nanoparticles; parathyroid hormone; poly(lactide-co-glycolide); scaffolds
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MDPI and ACS Style

Gentile, P.; Nandagiri, V.K.; Pabari, R.; Daly, J.; Tonda-Turo, C.; Ciardelli, G.; Ramtoola, Z. Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration. Int. J. Mol. Sci. 2015, 16, 20492-20510. https://doi.org/10.3390/ijms160920492

AMA Style

Gentile P, Nandagiri VK, Pabari R, Daly J, Tonda-Turo C, Ciardelli G, Ramtoola Z. Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration. International Journal of Molecular Sciences. 2015; 16(9):20492-20510. https://doi.org/10.3390/ijms160920492

Chicago/Turabian Style

Gentile, Piergiorgio, Vijay Kumar Nandagiri, Ritesh Pabari, Jacqueline Daly, Chiara Tonda-Turo, Gianluca Ciardelli, and Zebunnissa Ramtoola. 2015. "Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration" International Journal of Molecular Sciences 16, no. 9: 20492-20510. https://doi.org/10.3390/ijms160920492

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