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Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration

School of Clinical Dentistry, University of Sheffield, Sheffield S10 2TA, UK
Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin 10129, Italy
School of Pharmacy, Royal College of Surgeons in Ireland, Dublin 2, Ireland
Division of Biology, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin 2, Ireland
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Bing Yan
Int. J. Mol. Sci. 2015, 16(9), 20492-20510;
Received: 21 July 2015 / Revised: 5 August 2015 / Accepted: 7 August 2015 / Published: 28 August 2015
(This article belongs to the Collection Bioactive Nanoparticles)
PDF [1510 KB, uploaded 28 August 2015]


Biodegradable poly(lactide-co-glycolide) (PLGA) nanoparticles, containing human parathyroid hormone (PTH (1–34)), prepared by a modified double emulsion-solvent diffusion-evaporation method, were incorporated in porous freeze-dried chitosan-gelatin (CH-G) scaffolds. The PTH-loaded nanoparticles (NPTH) were characterised in terms of morphology, size, protein loading, release kinetics and in vitro assessment of biological activity of released PTH and cytocompatibility studies against clonal human osteoblast (hFOB) cells. Structural integrity of incorporated and released PTH from nanoparticles was found to be intact by using Tris-tricine SDS-PAGE. In vitro PTH release kinetics from PLGA nanoparticles were characterised by a burst release followed by a slow release phase for 3–4 weeks. The released PTH was biologically active as evidenced by the stimulated release of cyclic AMP from hFOB cells as well as increased mineralisation studies. in vitro and cell studies demonstrated that the PTH bioactivity was maintained during the fabrication of PLGA nanoparticles and upon release. Finally, a content of 33.3% w/w NPTHs was incorporated in CH-G scaffolds, showing an intermittent release during the first 10 days and, followed by a controlled release over 28 days of observation time. The increased expression of Alkaline Phosphatase levels on hFOB cells further confirmed the activity of intermittently released PTH from scaffolds. View Full-Text
Keywords: bone tissue; nanoparticles; parathyroid hormone; poly(lactide-co-glycolide); scaffolds bone tissue; nanoparticles; parathyroid hormone; poly(lactide-co-glycolide); scaffolds

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Gentile, P.; Nandagiri, V.K.; Pabari, R.; Daly, J.; Tonda-Turo, C.; Ciardelli, G.; Ramtoola, Z. Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration. Int. J. Mol. Sci. 2015, 16, 20492-20510.

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