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Int. J. Mol. Sci. 2015, 16(7), 16034-16052;

Effect of rhBMP-2 Immobilized Anorganic Bovine Bone Matrix on Bone Regeneration

Department of Prosthodontics, Dental Research Institute, Biomedical Research Institute, Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, YangSan 676-870, Korea
Advanced Prosthodontics, Postgraduate School of Clinical Dentistry, Institute for Clinical Dental Research, Korea University, Seoul 152-703, Korea
Research Development Institute, Cowellmedi Co., Ltd., Busan 617-801, Korea
Department of Orthopedic Surgery & Rare Diseases Institute, Korea University Medical Center, Guro Hospital, Seoul 152-703, Korea
Author to whom correspondence should be addressed.
Academic Editor: Mohamed N. Rahaman
Received: 22 April 2015 / Revised: 24 June 2015 / Accepted: 7 July 2015 / Published: 14 July 2015
(This article belongs to the Special Issue Biomaterials for Tissue Engineering)
Full-Text   |   PDF [12495 KB, uploaded 14 July 2015]   |  


Anorganic bovine bone matrix (Bio-Oss®) has been used for a long time for bone graft regeneration, but has poor osteoinductive capability. The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) has been suggested to overcome this limitation of Bio-Oss®. In the present study, heparin-mediated rhBMP-2 was combined with Bio-Oss® in animal experiments to investigate bone formation performance; heparin was used to control rhBMP-2 release. Two calvarial defects (8 mm diameter) were formed in a white rabbit model and then implanted or not (controls) with Bio-Oss® or BMP-2/Bio-Oss®. The Bio-Oss® and BMP-2/Bio-Oss® groups had significantly greater new bone areas (expressed as percentages of augmented areas) than the non-implanted controls at four and eight weeks after surgery, and the BMP-2/Bio-Oss® group (16.50 ± 2.87 (n = 6)) had significantly greater new bone areas than the Bio-Oss® group (9.43 ± 3.73 (n = 6)) at four weeks. These findings suggest that rhBMP-2 treated heparinized Bio-Oss® markedly enhances bone regeneration. View Full-Text
Keywords: bone regeneration; anorganic bovine bone; Bio-Oss®; rhBMP-2 bone regeneration; anorganic bovine bone; Bio-Oss®; rhBMP-2

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Huh, J.-B.; Yang, J.-J.; Choi, K.-H.; Bae, J.H.; Lee, J.-Y.; Kim, S.-E.; Shin, S.-W. Effect of rhBMP-2 Immobilized Anorganic Bovine Bone Matrix on Bone Regeneration. Int. J. Mol. Sci. 2015, 16, 16034-16052.

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