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The Effect of Diagnostic Absorbed Doses from 131I on Human Thyrocytes in Vitro

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 93-338 Lodz, Poland
Department of Endocrinology and Metabolic Diseases, Polish Mother's Memorial Hospital—Research Institute, 93-338 Lodz, Poland
Department of Neurology, Polish Mother's Memorial Hospital—Research Institute, 93-338 Lodz, Poland
Food Science Department, Medical University of Lodz, 90-151 Lodz, Poland
Department of Oncological Endocrinology and Nuclear Medicine, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, 02-781 Warsaw, Poland
Department of Biopharmacy, Medical University of Lodz, 90-151 Lodz, Poland
Department of Hematology, Institute of Hematology and Transfusion Medicine, 02-776 Warsaw, Poland
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Terrence Piva
Int. J. Mol. Sci. 2015, 16(7), 14608-14622;
Received: 10 April 2015 / Revised: 27 May 2015 / Accepted: 3 June 2015 / Published: 29 June 2015
(This article belongs to the Collection Radiation Toxicity in Cells)
PDF [1542 KB, uploaded 29 June 2015]


Background: Administration of diagnostic activities of 131I, performed in order to detect thyroid remnants after surgery and/or thyroid cancer recurrence/metastases, may lead to reduction of iodine uptake. This phenomenon is called “thyroid stunning”. We estimated radiation absorbed dose-dependent changes in genetic material, in particular in sodium iodide symporter (NIS) gene promoter, and NIS protein level in human thyrocytes (HT). Materials and Methods: We used unmodified HT isolated from patients subjected to thyroidectomy exposed to 131I in culture. The different 131I activities applied were calculated to result in absorbed doses of 5, 10, and 20 Gy. Results: According to flow cytometry analysis and comet assay, 131I did not influence the HT viability in culture. Temporary increase of 8-oxo-dG concentration in HT directly after 24 h (p < 0.05) and increase in the number of AP-sites 72 h after termination of exposition to 20 Gy dose (p < 0.0001) were observed. The signs of dose-dependent DNA damage were not associated with essential changes in the NIS expression on mRNA and protein levels. Conclusions: Our observation constitutes a first attempt to evaluate the effect of the absorbed dose of 131I on HT. The results have not confirmed the theory that the “thyroid stunning” reduces the NIS protein synthesis. View Full-Text
Keywords: thyroid gland; 131I; thyroid stunning thyroid gland; 131I; thyroid stunning

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Adamczewski, Z.; Stasiołek, M.; Karwowski, B.; Dedecjus, M.; Orszulak-Michalak, D.; Merecz, A.; Śliwka, P.W.; Puła, B.; Lewiński, A. The Effect of Diagnostic Absorbed Doses from 131I on Human Thyrocytes in Vitro. Int. J. Mol. Sci. 2015, 16, 14608-14622.

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