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Int. J. Mol. Sci. 2015, 16(7), 14318-14337;

Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha 24144, Qatar
Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10022, USA
UMR_I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, Université de Reims Champagne-Ardenne, BP-1039-Reims Cedex 2, 51687 Reims, France
UGSF, UMR CNRS 8576, équipe Régulation des Signaux de Division, Université de Lille, Sciences et Technologies, 59009 Villeneuve d'Ascq, France
Laboratory of Ecotoxicology UPRES EA 3222, IFRMP 23, University of Le Havre, 76058 Le Havre, France
Author to whom correspondence should be addressed.
Academic Editor: Tuhin Subhra Santra
Received: 24 March 2015 / Revised: 6 May 2015 / Accepted: 14 May 2015 / Published: 24 June 2015
(This article belongs to the Special Issue Single Cell Analysis in Biotechnology and Systems Biology)
Full-Text   |   PDF [3329 KB, uploaded 24 June 2015]   |  


The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation. View Full-Text
Keywords: multi-drug resistance; P-glycoprotein; cell volume; flow cytometer; MCF7 multi-drug resistance; P-glycoprotein; cell volume; flow cytometer; MCF7

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Pasquier, J.; Rioult, D.; Abu-Kaoud, N.; Hoarau-Véchot, J.; Marin, M.; Foll, F.L. Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry. Int. J. Mol. Sci. 2015, 16, 14318-14337.

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